Extraction of myosin light chains and actin from bovine cardiac muscle acetone powder

Julian A. Barden; Patricia Symons; John A. Cartmill; Masao Miki; Brett D. Hambly

doi:10.1016/0003-2697(86)90552-X

Extraction of myosin light chains and actin from bovine cardiac muscle acetone powder

Julian A. Barden^*, Patricia Symons, John A. Cartmill, Masao Miki, Brett D. Hambly

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A joint preparation of the two myosin light chains and actin from bovine cardiac muscle acetone powder is described. There is a significant improvement in the ease of purification, while the yield of the myosin light chains equals the best yields obtained from the use of estabished techniques. The actin yield greatly exceeds that obtained in an earlier published report.

Original language	English
Pages (from-to)	288-293
Number of pages	6
Journal	Analytical Biochemistry
Volume	158
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(86)90552-X
Publication status	Published - 1 Nov 1986
Externally published	Yes

Keywords

actin
cardiac muscle
gel chromatography
HPLC
myosin light chains
protein purification

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10.1016/0003-2697(86)90552-X

Cite this

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title = "Extraction of myosin light chains and actin from bovine cardiac muscle acetone powder",

abstract = "A joint preparation of the two myosin light chains and actin from bovine cardiac muscle acetone powder is described. There is a significant improvement in the ease of purification, while the yield of the myosin light chains equals the best yields obtained from the use of estabished techniques. The actin yield greatly exceeds that obtained in an earlier published report.",

keywords = "actin, cardiac muscle, gel chromatography, HPLC, myosin light chains, protein purification",

author = "Barden, {Julian A.} and Patricia Symons and Cartmill, {John A.} and Masao Miki and Hambly, {Brett D.}",

year = "1986",

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AU - Barden, Julian A.

AU - Symons, Patricia

AU - Cartmill, John A.

AU - Miki, Masao

AU - Hambly, Brett D.

PY - 1986/11/1

Y1 - 1986/11/1

N2 - A joint preparation of the two myosin light chains and actin from bovine cardiac muscle acetone powder is described. There is a significant improvement in the ease of purification, while the yield of the myosin light chains equals the best yields obtained from the use of estabished techniques. The actin yield greatly exceeds that obtained in an earlier published report.

AB - A joint preparation of the two myosin light chains and actin from bovine cardiac muscle acetone powder is described. There is a significant improvement in the ease of purification, while the yield of the myosin light chains equals the best yields obtained from the use of estabished techniques. The actin yield greatly exceeds that obtained in an earlier published report.

KW - actin

KW - cardiac muscle

KW - gel chromatography

KW - HPLC

KW - myosin light chains

KW - protein purification

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DO - 10.1016/0003-2697(86)90552-X

M3 - Article

C2 - 3812973

AN - SCOPUS:0022972660

SN - 0003-2697

VL - 158

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EP - 293

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Extraction of myosin light chains and actin from bovine cardiac muscle acetone powder

Abstract

Keywords

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