Extraction of myosin light chains and actin from bovine cardiac muscle acetone powder

Julian A. Barden*, Patricia Symons, John A. Cartmill, Masao Miki, Brett D. Hambly

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A joint preparation of the two myosin light chains and actin from bovine cardiac muscle acetone powder is described. There is a significant improvement in the ease of purification, while the yield of the myosin light chains equals the best yields obtained from the use of estabished techniques. The actin yield greatly exceeds that obtained in an earlier published report.

Original languageEnglish
Pages (from-to)288-293
Number of pages6
JournalAnalytical Biochemistry
Volume158
Issue number2
DOIs
Publication statusPublished - 1 Nov 1986
Externally publishedYes

Keywords

  • actin
  • cardiac muscle
  • gel chromatography
  • HPLC
  • myosin light chains
  • protein purification

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