TY - JOUR
T1 - Extraction of nucleic acids from agarose gels
AU - Langridge, J.
AU - Langridge, P.
AU - Bergquist, P. L.
PY - 1980/3/15
Y1 - 1980/3/15
N2 - A rapid, simple and versatile method is described for the extraction from agarose gels of small plasmid molecules and DNA fragments generated by restriction endonucleases. The method may be used also for the extraction of RNA from agarose-urea gels. It is based on the partitioning of nucleic acid molecules into 1-butanol as their quaternary ammonium salts, leaving the neutral agarose in the aqueous phase. The nucleic acid is then recovered as the sodium salt by partition back into an aqueous phase. Nucleic acid samples were found to be unaffected by the treatment, as judged by their ability to be ligated, transformed, nick-translated, and used in an in vitro protein-synthesizing system.
AB - A rapid, simple and versatile method is described for the extraction from agarose gels of small plasmid molecules and DNA fragments generated by restriction endonucleases. The method may be used also for the extraction of RNA from agarose-urea gels. It is based on the partitioning of nucleic acid molecules into 1-butanol as their quaternary ammonium salts, leaving the neutral agarose in the aqueous phase. The nucleic acid is then recovered as the sodium salt by partition back into an aqueous phase. Nucleic acid samples were found to be unaffected by the treatment, as judged by their ability to be ligated, transformed, nick-translated, and used in an in vitro protein-synthesizing system.
UR - http://www.scopus.com/inward/record.url?scp=0018836524&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(80)90266-3
DO - 10.1016/0003-2697(80)90266-3
M3 - Article
C2 - 6155806
AN - SCOPUS:0018836524
SN - 0003-2697
VL - 103
SP - 264
EP - 271
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -