Abstract

The treatment depth of existing photodynamic therapy (PDT) is limited because of the absorption of visible excitation light in biological tissue. It can be augmented by means of upconversion nanoparticles (UCNPs) transforming deep-penetrating near-infrared (NIR) light to visible light, exciting PDT drugs. We report here a facile strategy to assemble such PDT nanocomposites functionalized for cancer targeting, based on coating of the UCNPs with a silica layer encapsulating the Rose Bengal photosensitizer and bioconjugation to antibodies through a bifunctional fusion protein consisting of a solid-binding peptide linker genetically fused to Streptococcus Protein G′. The fusion protein (Linker-Protein G) mediates the functionalization of silica-coated UCNPs with cancer cell antibodies, allowing for specific target recognition and delivery. The resulting nanocomposites were shown to target cancer cells specifically, generate intracellular reactive oxygen species under 980 nm excitation, and induce NIR-triggered phototoxicity to suppress cancer cell growth in vitro.

LanguageEnglish
Pages11945-11953
Number of pages9
JournalACS Applied Materials and Interfaces
Volume8
Issue number19
DOIs
Publication statusPublished - 18 May 2016

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Photodynamic therapy
Nanoparticles
Proteins
Imaging techniques
Silicon Dioxide
Nanocomposites
Fusion reactions
Cells
Antibodies
Infrared radiation
Rose Bengal
Photosensitizing Agents
Silica
Cell growth
Photosensitizers
Reactive Oxygen Species
Tissue
Peptides
Coatings
Pharmaceutical Preparations

Keywords

  • luminescence resonance energy transfer
  • photodynamic therapy
  • solid-binding peptides
  • targeted imaging
  • upconversion nanoparticles

Cite this

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title = "Facile assembly of functional upconversion nanoparticles for targeted cancer imaging and photodynamic therapy",
abstract = "The treatment depth of existing photodynamic therapy (PDT) is limited because of the absorption of visible excitation light in biological tissue. It can be augmented by means of upconversion nanoparticles (UCNPs) transforming deep-penetrating near-infrared (NIR) light to visible light, exciting PDT drugs. We report here a facile strategy to assemble such PDT nanocomposites functionalized for cancer targeting, based on coating of the UCNPs with a silica layer encapsulating the Rose Bengal photosensitizer and bioconjugation to antibodies through a bifunctional fusion protein consisting of a solid-binding peptide linker genetically fused to Streptococcus Protein G′. The fusion protein (Linker-Protein G) mediates the functionalization of silica-coated UCNPs with cancer cell antibodies, allowing for specific target recognition and delivery. The resulting nanocomposites were shown to target cancer cells specifically, generate intracellular reactive oxygen species under 980 nm excitation, and induce NIR-triggered phototoxicity to suppress cancer cell growth in vitro.",
keywords = "luminescence resonance energy transfer, photodynamic therapy, solid-binding peptides, targeted imaging, upconversion nanoparticles",
author = "Liuen Liang and Andrew Care and Run Zhang and Yiqing Lu and Packer, {Nicolle H.} and Anwar Sunna and Yi Qian and Zvyagin, {Andrei V.}",
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AU - Liang, Liuen

AU - Care, Andrew

AU - Zhang, Run

AU - Lu, Yiqing

AU - Packer, Nicolle H.

AU - Sunna, Anwar

AU - Qian, Yi

AU - Zvyagin, Andrei V.

PY - 2016/5/18

Y1 - 2016/5/18

N2 - The treatment depth of existing photodynamic therapy (PDT) is limited because of the absorption of visible excitation light in biological tissue. It can be augmented by means of upconversion nanoparticles (UCNPs) transforming deep-penetrating near-infrared (NIR) light to visible light, exciting PDT drugs. We report here a facile strategy to assemble such PDT nanocomposites functionalized for cancer targeting, based on coating of the UCNPs with a silica layer encapsulating the Rose Bengal photosensitizer and bioconjugation to antibodies through a bifunctional fusion protein consisting of a solid-binding peptide linker genetically fused to Streptococcus Protein G′. The fusion protein (Linker-Protein G) mediates the functionalization of silica-coated UCNPs with cancer cell antibodies, allowing for specific target recognition and delivery. The resulting nanocomposites were shown to target cancer cells specifically, generate intracellular reactive oxygen species under 980 nm excitation, and induce NIR-triggered phototoxicity to suppress cancer cell growth in vitro.

AB - The treatment depth of existing photodynamic therapy (PDT) is limited because of the absorption of visible excitation light in biological tissue. It can be augmented by means of upconversion nanoparticles (UCNPs) transforming deep-penetrating near-infrared (NIR) light to visible light, exciting PDT drugs. We report here a facile strategy to assemble such PDT nanocomposites functionalized for cancer targeting, based on coating of the UCNPs with a silica layer encapsulating the Rose Bengal photosensitizer and bioconjugation to antibodies through a bifunctional fusion protein consisting of a solid-binding peptide linker genetically fused to Streptococcus Protein G′. The fusion protein (Linker-Protein G) mediates the functionalization of silica-coated UCNPs with cancer cell antibodies, allowing for specific target recognition and delivery. The resulting nanocomposites were shown to target cancer cells specifically, generate intracellular reactive oxygen species under 980 nm excitation, and induce NIR-triggered phototoxicity to suppress cancer cell growth in vitro.

KW - luminescence resonance energy transfer

KW - photodynamic therapy

KW - solid-binding peptides

KW - targeted imaging

KW - upconversion nanoparticles

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