Facile peptides functionalization of lanthanide-based nanocrystals through phosphorylation tethering for efficient in vivo NIR-to-NIR bioimaging

Chi Yao, Peiyuan Wang, Rui Wang, Lei Zhou, Ahmed Mohamed El-Toni, Yiqing Lu, Xiaomin Li, Fan Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Peptide modification of nanoparticles is a challenging task for bioapplications. Here, we show that noncovalent surface engineering based on ligand exchange of peptides for lanthanide based upconversion and downconversion near-infrared (NIR) luminescent nanoparticles can be efficiently realized by modifying the hydroxyl functional group of a side grafted serine of peptides into a phosphate group (phosphorylation). By using the phosphorylated peptide with the arginine-glycine-aspartic acid (RGD) targeting motifs as typical examples, the modification allows improving the selectivity, sensitivity, and signal-to-noise ratio for the cancer targeting and bioimaging and reducing the toxicity derived from nonspecific interactions of nanoparticles with cells. The in vivo NIR bioimaging signal could even be detected at low injection amounts down to 20 μg per animal.

Original languageEnglish
Pages (from-to)1930-1936
Number of pages7
JournalAnalytical Chemistry
Volume88
Issue number3
DOIs
Publication statusPublished - 2 Feb 2016

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