Fluorescence analysis detects gp60 subtype diversity in Cryptosporidium infections

L. S. Waldron, M. L. Power

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Ninety percent of human cryptosporidiosis infections are attributed to two species; the anthroponotic Cryptosporidium hominis and the zoonotic Cryptosporidium parvum. Sequence analysis of the hypervariable gp60 gene, which is used to classify Cryptosporidium to the subtype level, has highlighted extensive intra-species diversity within both C. hominis and C. parvum. The gp60 has also facilitated contamination source tracking and increased understanding of the epidemiology of cryptosporidiosis. Two surface glycoproteins, the gp40 and gp15 are encoded in the gp60 gene; both are exposed to the hosts' immune system and play a pivotal role in the disease initiation process. The extent of genetic diversity observed within the gp60 would support the hypotheses of significant selection pressure placed on the gp40 and gp15. This study used a dual fluorescent terminal-restriction fragment length polymorphism (T-RFLP) analysis to investigate the genetic diversity of Cryptosporidium subtype populations in a single host infection. Terminal-RFLP showed subtype variation within one human Cryptosporidium sample and mouse samples from seven consecutive passages with C. parvum. Furthermore, this was the first study to show that differences in the ratio of subtype populations occur between infections. T-RFLP has provided a novel platform to study infection populations and to begin to investigate the impact of the hosts' immune system on the gp60 gene.

LanguageEnglish
Pages1388-1395
Number of pages8
JournalInfection, Genetics and Evolution
Volume11
Issue number6
DOIs
Publication statusPublished - Aug 2011

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Cryptosporidium
Cryptosporidium parvum
fluorescence
Cryptosporidium hominis
Fluorescence
Restriction Fragment Length Polymorphisms
Cryptosporidiosis
cryptosporidiosis
restriction fragment length polymorphism
immune system
Infection
infection
gene
Immune System
polymorphism
Population
Genes
genetic variation
genes
Membrane Glycoproteins

Cite this

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title = "Fluorescence analysis detects gp60 subtype diversity in Cryptosporidium infections",
abstract = "Ninety percent of human cryptosporidiosis infections are attributed to two species; the anthroponotic Cryptosporidium hominis and the zoonotic Cryptosporidium parvum. Sequence analysis of the hypervariable gp60 gene, which is used to classify Cryptosporidium to the subtype level, has highlighted extensive intra-species diversity within both C. hominis and C. parvum. The gp60 has also facilitated contamination source tracking and increased understanding of the epidemiology of cryptosporidiosis. Two surface glycoproteins, the gp40 and gp15 are encoded in the gp60 gene; both are exposed to the hosts' immune system and play a pivotal role in the disease initiation process. The extent of genetic diversity observed within the gp60 would support the hypotheses of significant selection pressure placed on the gp40 and gp15. This study used a dual fluorescent terminal-restriction fragment length polymorphism (T-RFLP) analysis to investigate the genetic diversity of Cryptosporidium subtype populations in a single host infection. Terminal-RFLP showed subtype variation within one human Cryptosporidium sample and mouse samples from seven consecutive passages with C. parvum. Furthermore, this was the first study to show that differences in the ratio of subtype populations occur between infections. T-RFLP has provided a novel platform to study infection populations and to begin to investigate the impact of the hosts' immune system on the gp60 gene.",
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Fluorescence analysis detects gp60 subtype diversity in Cryptosporidium infections. / Waldron, L. S.; Power, M. L.

In: Infection, Genetics and Evolution, Vol. 11, No. 6, 08.2011, p. 1388-1395.

Research output: Contribution to journalArticleResearchpeer-review

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