TY - JOUR
T1 - Fluorescence detection of Fe3+ ions in aqueous solution and living cells based on a high selectivity and sensitivity chemosensor
AU - Jia, Hongmin
AU - Gao, Xue
AU - Shi, Yu
AU - Sayyadi, Nima
AU - Zhang, Zhiqiang
AU - Zhao, Qi
AU - Meng, Qingtao
AU - Zhang, Run
PY - 2015/10/5
Y1 - 2015/10/5
N2 - Although ferric ion (Fe3+) performs critical roles in diverse biochemical processes in living systems, its physiological and pathophysiological functions have not been fully explored due to the lack of methods for quantification of Fe3+ ions in biological system. In this work, a highly sensitive and selective fluorescence chemosensor, L, was developed for the detection of Fe3+ ions in aqueous solution and in living cells. L was facile synthesized by one step reaction and well characterized by NMR, API-ES, FT-IR, and elementary analysis. The prepared chemosensor displayed excellent selectivity for Fe3+ ions detection over a wide range of tested metal ions. In the present of Fe3+ ions, the strong green fluorescence of L was substantially quenched. The 1:1 stoichiometry of the complexation was confirmed by a Job's plot. The association constant (Ka) of L with Fe3+ was evaluated using the Benesi-Hildebrand method and was found to be 1.36 × 104 M-1. The MTT assay determined that L exhibits low cytotoxicity toward living cells. Confocal imaging and flow cytometry studies showed that L is readily interiorized by MDA-MB-231 cells through an energy-dependent pathway and could be used to detect of Fe3+ ions in living cells.
AB - Although ferric ion (Fe3+) performs critical roles in diverse biochemical processes in living systems, its physiological and pathophysiological functions have not been fully explored due to the lack of methods for quantification of Fe3+ ions in biological system. In this work, a highly sensitive and selective fluorescence chemosensor, L, was developed for the detection of Fe3+ ions in aqueous solution and in living cells. L was facile synthesized by one step reaction and well characterized by NMR, API-ES, FT-IR, and elementary analysis. The prepared chemosensor displayed excellent selectivity for Fe3+ ions detection over a wide range of tested metal ions. In the present of Fe3+ ions, the strong green fluorescence of L was substantially quenched. The 1:1 stoichiometry of the complexation was confirmed by a Job's plot. The association constant (Ka) of L with Fe3+ was evaluated using the Benesi-Hildebrand method and was found to be 1.36 × 104 M-1. The MTT assay determined that L exhibits low cytotoxicity toward living cells. Confocal imaging and flow cytometry studies showed that L is readily interiorized by MDA-MB-231 cells through an energy-dependent pathway and could be used to detect of Fe3+ ions in living cells.
KW - Chemosensor
KW - Ferric ion
KW - Detection
KW - Fluorescence imaging
KW - Flow cytometry
UR - http://www.scopus.com/inward/record.url?scp=84929359130&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2015.04.111
DO - 10.1016/j.saa.2015.04.111
M3 - Article
C2 - 25985133
AN - SCOPUS:84929359130
SN - 1386-1425
VL - 149
SP - 674
EP - 681
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
ER -