Fluorescence lifetime imaging in drug delivery research

[Graphical abstract presents]

Once an exotic add-on to fluorescence microscopy for life science research, fluorescence lifetime imaging (FLIm) has become a powerful and increasingly utilised technique owing to its self-calibration nature, which affords superior quantification over conventional steady-state fluorescence imaging. This review focuses on the state-of-the-art implementation of FLIm related to the formulation, release, dosage, and mechanism of action of drugs aimed for innovative diagnostics and therapy. Quantitative measurements using FLIm have appeared instrumental for encapsulated drug delivery design, pharmacokinetics and pharmacodynamics, pathological investigations, early disease diagnosis, and evaluation of therapeutic efficacy. Attention is paid to the latest advances in lifetime-engineered nanomaterials and practical instrumentation, which begin to show preclinical and clinical translation potential beyond in vitro samples of cells and tissues. Finally, major challenges that need to be overcome in order to facilitate future perspectives are discussed.