Fluorescence recovery after photo-bleaching as a method to determine local diffusion coefficient in the stratum corneum

Yuri G. Anissimov; Xin Zhao; Michael S. Roberts; Andrei V. Zvyagin

doi:10.1016/j.ijpharm.2012.01.055

Fluorescence recovery after photo-bleaching as a method to determine local diffusion coefficient in the stratum corneum

Yuri G. Anissimov^*, Xin Zhao, Michael S. Roberts, Andrei V. Zvyagin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Fluorescence recovery after photo-bleaching experiments were performed in human stratum corneum in vitro. Fluorescence multiphoton tomography was used, which allowed the dimensions of the photobleached volume to be at the micron scale and located fully within the lipid phase of the stratum corneum. Analysis of the fluorescence recovery data with simplified mathematical models yielded the diffusion coefficient of small molecular weight organic fluorescent dye Rhodamine B in the stratum corneum lipid phase of about (3-6) × 10 ^-9 cm² s^-1. It was concluded that the presented method can be used for detailed analysis of localised diffusion coefficients in the stratum corneum phases for various fluorescent probes.

Original language	English
Pages (from-to)	93-97
Number of pages	5
Journal	International Journal of Pharmaceutics
Volume	435
Issue number	1
DOIs	https://doi.org/10.1016/j.ijpharm.2012.01.055
Publication status	Published - 1 Oct 2012

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10.1016/j.ijpharm.2012.01.055

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abstract = "Fluorescence recovery after photo-bleaching experiments were performed in human stratum corneum in vitro. Fluorescence multiphoton tomography was used, which allowed the dimensions of the photobleached volume to be at the micron scale and located fully within the lipid phase of the stratum corneum. Analysis of the fluorescence recovery data with simplified mathematical models yielded the diffusion coefficient of small molecular weight organic fluorescent dye Rhodamine B in the stratum corneum lipid phase of about (3-6) × 10 -9 cm2 s-1. It was concluded that the presented method can be used for detailed analysis of localised diffusion coefficients in the stratum corneum phases for various fluorescent probes.",

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AU - Zhao, Xin

AU - Roberts, Michael S.

AU - Zvyagin, Andrei V.

PY - 2012/10/1

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AB - Fluorescence recovery after photo-bleaching experiments were performed in human stratum corneum in vitro. Fluorescence multiphoton tomography was used, which allowed the dimensions of the photobleached volume to be at the micron scale and located fully within the lipid phase of the stratum corneum. Analysis of the fluorescence recovery data with simplified mathematical models yielded the diffusion coefficient of small molecular weight organic fluorescent dye Rhodamine B in the stratum corneum lipid phase of about (3-6) × 10 -9 cm2 s-1. It was concluded that the presented method can be used for detailed analysis of localised diffusion coefficients in the stratum corneum phases for various fluorescent probes.

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Fluorescence recovery after photo-bleaching as a method to determine local diffusion coefficient in the stratum corneum

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