Fluoroimmunoassays for the detection of zearalenone in maize using CdTe/CdS/ZnS quantum dots

Fuyuan Zhang, Bing Liu, Wei Sheng, Yan Zhang, Qi Liu, Shijie Li, Shuo Wang*

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

CdTe/CdS/ZnS quantum dots (QDs) were synthesized in aqueous phase and conjugated with a new anti-zearalenone (ZEN) monoclonal antibody. Using this novel fluorescent probe, a fluoroimmunoassay (FLISA) and a rapid immunochromatographic strip (ICTS) were developed for the detection of ZEN in maize. Our proposed FLISA allowed for ZEN determination in the range of 0.038–0.977 ng/mL with an IC50 of 0.162 ng/mL and a limit of detection (LOD) of 0.012 ng/mL occurring in maize. The rapid ICTS had a visual LOD of 1.0 ng/mL in buffer solution and 1.5 ng/mL in maize extract. These two QDs-based immunoassays were all successfully verified by commercial ELISA kits. The results confirmed that: firstly, the FLISA can be used as a robust method for the sensitive detection of ZEN; and secondly, the ICTS is ideally suited for rapidly screening large numbers of samples.

Original languageEnglish
Pages (from-to)421-428
Number of pages8
JournalFood Chemistry
Volume255
DOIs
Publication statusPublished - 30 Jul 2018

Keywords

  • CdTe/CdS/ZnS
  • Fluoroimmunoassay
  • Immunochromatographic strip
  • Quantum dots
  • Zearalenone

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