Force measurements for cancer cells

Vivek Rajasekharan, Varun K A Sreenivasan, Brenda Farrell*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

During cytoskeleton remodeling, cancer cells generate force at the plasma membrane that originates from chemical motors (e.g., actin). This force (pN) and its time course reflect the on and off-rates of the motors. We describe the design and calibration of a force-measuring device (i.e., optical tweezers) that is used to monitor this force and its time course at the edge of a cell, with particular emphasis on the temporal resolution of the instrument.

Original languageEnglish
Title of host publicationCancer nanotechnology
Subtitle of host publicationmethods and protocols
EditorsReema Zeineldin
Place of PublicationNew York, NY
PublisherHumana Press
Chapter12
Pages195-228
Number of pages34
ISBN (Electronic)9781493966462
ISBN (Print)9781493966448
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1530
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Actin
  • Cell mobility
  • Cytoskeleton
  • Filopodium
  • Membrane force
  • Membrane tube
  • Motor kinetics
  • Noise of signal
  • Optical tweezers
  • Time constant

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  • Cite this

    Rajasekharan, V., Sreenivasan, V. K. A., & Farrell, B. (2017). Force measurements for cancer cells. In R. Zeineldin (Ed.), Cancer nanotechnology: methods and protocols (pp. 195-228). (Methods in Molecular Biology; Vol. 1530). New York, NY: Humana Press. https://doi.org/10.1007/978-1-4939-6646-2_12