Force measurements for cancer cells

Vivek Rajasekharan; Varun K A Sreenivasan; Brenda Farrell

doi:10.1007/978-1-4939-6646-2_12

Force measurements for cancer cells

Vivek Rajasekharan, Varun K A Sreenivasan, Brenda Farrell^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

During cytoskeleton remodeling, cancer cells generate force at the plasma membrane that originates from chemical motors (e.g., actin). This force (pN) and its time course reflect the on and off-rates of the motors. We describe the design and calibration of a force-measuring device (i.e., optical tweezers) that is used to monitor this force and its time course at the edge of a cell, with particular emphasis on the temporal resolution of the instrument.

Original language	English
Title of host publication	Cancer nanotechnology
Subtitle of host publication	methods and protocols
Editors	Reema Zeineldin
Place of Publication	New York, NY
Publisher	Humana Press
Chapter	12
Pages	195-228
Number of pages	34
ISBN (Electronic)	9781493966462
ISBN (Print)	9781493966448
DOIs	https://doi.org/10.1007/978-1-4939-6646-2_12
Publication status	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1530
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Actin
Cell mobility
Cytoskeleton
Filopodium
Membrane force
Membrane tube
Motor kinetics
Noise of signal
Optical tweezers
Time constant

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10.1007/978-1-4939-6646-2_12

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year = "2017",

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language = "English",

isbn = "9781493966448",

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T1 - Force measurements for cancer cells

AU - Rajasekharan, Vivek

AU - Sreenivasan, Varun K A

AU - Farrell, Brenda

PY - 2017

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AB - During cytoskeleton remodeling, cancer cells generate force at the plasma membrane that originates from chemical motors (e.g., actin). This force (pN) and its time course reflect the on and off-rates of the motors. We describe the design and calibration of a force-measuring device (i.e., optical tweezers) that is used to monitor this force and its time course at the edge of a cell, with particular emphasis on the temporal resolution of the instrument.

KW - Actin

KW - Cell mobility

KW - Cytoskeleton

KW - Filopodium

KW - Membrane force

KW - Membrane tube

KW - Motor kinetics

KW - Noise of signal

KW - Optical tweezers

KW - Time constant

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AN - SCOPUS:85011556592

SN - 9781493966448

T3 - Methods in Molecular Biology

SP - 195

EP - 228

BT - Cancer nanotechnology

A2 - Zeineldin, Reema

PB - Humana Press

CY - New York, NY

ER -

Force measurements for cancer cells

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