Force measurements for cancer cells

Vivek Rajasekharan, Varun K A Sreenivasan, Brenda Farrell*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


    During cytoskeleton remodeling, cancer cells generate force at the plasma membrane that originates from chemical motors (e.g., actin). This force (pN) and its time course reflect the on and off-rates of the motors. We describe the design and calibration of a force-measuring device (i.e., optical tweezers) that is used to monitor this force and its time course at the edge of a cell, with particular emphasis on the temporal resolution of the instrument.

    Original languageEnglish
    Title of host publicationCancer nanotechnology
    Subtitle of host publicationmethods and protocols
    EditorsReema Zeineldin
    Place of PublicationNew York, NY
    PublisherHumana Press
    Number of pages34
    ISBN (Electronic)9781493966462
    ISBN (Print)9781493966448
    Publication statusPublished - 2017

    Publication series

    NameMethods in Molecular Biology
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029


    • Actin
    • Cell mobility
    • Cytoskeleton
    • Filopodium
    • Membrane force
    • Membrane tube
    • Motor kinetics
    • Noise of signal
    • Optical tweezers
    • Time constant


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