Abstract
The Gouldian finch (Erythrura gouldiae) went through a huge population decline in the last century. To address questions on conservation genetics, ecology and evolutionary biology we characterised 120 microsatellite markers (30 Z-linked) in this species using four methods: designing primers from the reference genome of a confamilial species (the zebra finch Taeniopygia guttata, n = 15); Sanger (n = 47) and 454-sequencing (n = 5) of microsatellite-enriched Gouldian finch libraries; and characterisation of cross-species markers (n = 53). We also identified 1992 primer-designable microsatellites for this species.
| Language | English |
|---|---|
| Pages | 899-907 |
| Number of pages | 9 |
| Journal | Conservation Genetics Resources |
| Volume | 7 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Dec 2015 |
Fingerprint
Keywords
- Estrildidae
- Erythrura gouldiae
- Gouldian finch
- Microsatellite
- Next-generation sequencing
- Sex linkage
- Cross-species markers
Cite this
}
Four-way development of microsatellite markers for the Gouldian finch (Erythrura gouldiae). / Kim, Kang Wook; Zhang, Hanyuan; Horsburgh, Gavin J.; Bird, Susannah; Dawson, Deborah A.; Griffith, Simon C.; Burke, Terry.
In: Conservation Genetics Resources, Vol. 7, No. 4, 01.12.2015, p. 899-907.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Four-way development of microsatellite markers for the Gouldian finch (Erythrura gouldiae)
AU - Kim, Kang Wook
AU - Zhang, Hanyuan
AU - Horsburgh, Gavin J.
AU - Bird, Susannah
AU - Dawson, Deborah A.
AU - Griffith, Simon C.
AU - Burke, Terry
PY - 2015/12/1
Y1 - 2015/12/1
N2 - The Gouldian finch (Erythrura gouldiae) went through a huge population decline in the last century. To address questions on conservation genetics, ecology and evolutionary biology we characterised 120 microsatellite markers (30 Z-linked) in this species using four methods: designing primers from the reference genome of a confamilial species (the zebra finch Taeniopygia guttata, n = 15); Sanger (n = 47) and 454-sequencing (n = 5) of microsatellite-enriched Gouldian finch libraries; and characterisation of cross-species markers (n = 53). We also identified 1992 primer-designable microsatellites for this species.
AB - The Gouldian finch (Erythrura gouldiae) went through a huge population decline in the last century. To address questions on conservation genetics, ecology and evolutionary biology we characterised 120 microsatellite markers (30 Z-linked) in this species using four methods: designing primers from the reference genome of a confamilial species (the zebra finch Taeniopygia guttata, n = 15); Sanger (n = 47) and 454-sequencing (n = 5) of microsatellite-enriched Gouldian finch libraries; and characterisation of cross-species markers (n = 53). We also identified 1992 primer-designable microsatellites for this species.
KW - Estrildidae
KW - Erythrura gouldiae
KW - Gouldian finch
KW - Microsatellite
KW - Next-generation sequencing
KW - Sex linkage
KW - Cross-species markers
UR - http://www.scopus.com/inward/record.url?scp=84947608590&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/arc/DP130100418
U2 - 10.1007/s12686-015-0498-3
DO - 10.1007/s12686-015-0498-3
M3 - Article
VL - 7
SP - 899
EP - 907
JO - Conservation Genetics Resources
T2 - Conservation Genetics Resources
JF - Conservation Genetics Resources
SN - 1877-7252
IS - 4
ER -