Four-way development of microsatellite markers for the Gouldian finch (Erythrura gouldiae)

Kang Wook Kim; Hanyuan Zhang; Gavin J. Horsburgh; Susannah Bird; Deborah A. Dawson; Simon C. Griffith; Terry Burke

doi:10.1007/s12686-015-0498-3

Four-way development of microsatellite markers for the Gouldian finch (Erythrura gouldiae)

Kang Wook Kim^*, Hanyuan Zhang, Gavin J. Horsburgh, Susannah Bird, Deborah A. Dawson, Simon C. Griffith, Terry Burke

^*Corresponding author for this work

Department of Biological Sciences

Research output: Contribution to journal › Article

2 Citations (Scopus)

Abstract

The Gouldian finch (Erythrura gouldiae) went through a huge population decline in the last century. To address questions on conservation genetics, ecology and evolutionary biology we characterised 120 microsatellite markers (30 Z-linked) in this species using four methods: designing primers from the reference genome of a confamilial species (the zebra finch Taeniopygia guttata, n = 15); Sanger (n = 47) and 454-sequencing (n = 5) of microsatellite-enriched Gouldian finch libraries; and characterisation of cross-species markers (n = 53). We also identified 1992 primer-designable microsatellites for this species.

Original language	English
Pages (from-to)	899-907
Number of pages	9
Journal	Conservation Genetics Resources
Volume	7
Issue number	4
DOIs	https://doi.org/10.1007/s12686-015-0498-3
Publication status	Published - 1 Dec 2015

Keywords

Estrildidae
Erythrura gouldiae
Gouldian finch
Microsatellite
Next-generation sequencing
Sex linkage
Cross-species markers

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10.1007/s12686-015-0498-3

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1 Finished

The danger within: assessing the threats to an endangered finch from genetic incompatibility, limited dispersal and effective population size
Griffith, S., Rollins, L., Burke, T. & MQRES, M.
19/03/13 → 28/02/17
Project: Research

Cite this

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abstract = "The Gouldian finch (Erythrura gouldiae) went through a huge population decline in the last century. To address questions on conservation genetics, ecology and evolutionary biology we characterised 120 microsatellite markers (30 Z-linked) in this species using four methods: designing primers from the reference genome of a confamilial species (the zebra finch Taeniopygia guttata, n = 15); Sanger (n = 47) and 454-sequencing (n = 5) of microsatellite-enriched Gouldian finch libraries; and characterisation of cross-species markers (n = 53). We also identified 1992 primer-designable microsatellites for this species.",

keywords = "Estrildidae, Erythrura gouldiae, Gouldian finch, Microsatellite, Next-generation sequencing, Sex linkage, Cross-species markers",

author = "Kim, {Kang Wook} and Hanyuan Zhang and Horsburgh, {Gavin J.} and Susannah Bird and Dawson, {Deborah A.} and Griffith, {Simon C.} and Terry Burke",

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AU - Kim, Kang Wook

AU - Zhang, Hanyuan

AU - Horsburgh, Gavin J.

AU - Bird, Susannah

AU - Dawson, Deborah A.

AU - Griffith, Simon C.

AU - Burke, Terry

PY - 2015/12/1

Y1 - 2015/12/1

N2 - The Gouldian finch (Erythrura gouldiae) went through a huge population decline in the last century. To address questions on conservation genetics, ecology and evolutionary biology we characterised 120 microsatellite markers (30 Z-linked) in this species using four methods: designing primers from the reference genome of a confamilial species (the zebra finch Taeniopygia guttata, n = 15); Sanger (n = 47) and 454-sequencing (n = 5) of microsatellite-enriched Gouldian finch libraries; and characterisation of cross-species markers (n = 53). We also identified 1992 primer-designable microsatellites for this species.

AB - The Gouldian finch (Erythrura gouldiae) went through a huge population decline in the last century. To address questions on conservation genetics, ecology and evolutionary biology we characterised 120 microsatellite markers (30 Z-linked) in this species using four methods: designing primers from the reference genome of a confamilial species (the zebra finch Taeniopygia guttata, n = 15); Sanger (n = 47) and 454-sequencing (n = 5) of microsatellite-enriched Gouldian finch libraries; and characterisation of cross-species markers (n = 53). We also identified 1992 primer-designable microsatellites for this species.

KW - Estrildidae

KW - Erythrura gouldiae

KW - Gouldian finch

KW - Microsatellite

KW - Next-generation sequencing

KW - Sex linkage

KW - Cross-species markers

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Four-way development of microsatellite markers for the Gouldian finch (Erythrura gouldiae)

Abstract

Keywords

Access to Document

The danger within: assessing the threats to an endangered finch from genetic incompatibility, limited dispersal and effective population size

Cite this