Four-way development of microsatellite markers for the Gouldian finch (Erythrura gouldiae)

Kang Wook Kim*, Hanyuan Zhang, Gavin J. Horsburgh, Susannah Bird, Deborah A. Dawson, Simon C. Griffith, Terry Burke

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The Gouldian finch (Erythrura gouldiae) went through a huge population decline in the last century. To address questions on conservation genetics, ecology and evolutionary biology we characterised 120 microsatellite markers (30 Z-linked) in this species using four methods: designing primers from the reference genome of a confamilial species (the zebra finch Taeniopygia guttata, n = 15); Sanger (n = 47) and 454-sequencing (n = 5) of microsatellite-enriched Gouldian finch libraries; and characterisation of cross-species markers (n = 53). We also identified 1992 primer-designable microsatellites for this species.

Original languageEnglish
Pages (from-to)899-907
Number of pages9
JournalConservation Genetics Resources
Volume7
Issue number4
DOIs
Publication statusPublished - 1 Dec 2015

Keywords

  • Estrildidae
  • Erythrura gouldiae
  • Gouldian finch
  • Microsatellite
  • Next-generation sequencing
  • Sex linkage
  • Cross-species markers

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