From glycomics to functional glycomics of sugar chains

Identification of target proteins with functional changes using gene targeting mice and knock down cells of FUT8 as examples

Akihiro Kondo*, Wenzhe Li, Takatoshi Nakagawa, Miyako Nakano, Nobuto Koyama, Xiangchun Wang, Jianguo Gu, Eiji Miyoshi, Naoyuki Taniguchi

*Corresponding author for this work

Research output: Contribution to journalReview article

25 Citations (Scopus)

Abstract

Comprehensive analyses of proteins from cells and tissues are the most effective means of elucidating the expression patterns of individual disease-related proteins. On the other hand, the simultaneous separation and characterization of proteins by 1-DE or 2-DE followed by MS analysis are one of the fundamental approaches to proteomic analysis. However, these analyses do not permit the complete structural identification of glycans in glycoproteins or their structural characterization. Over half of all known proteins are glycosylated and glycan analyses of glycoproteins are requisite for fundamental proteomics studies. The analysis of glycan structural alterations in glycoproteins is becoming increasingly important in terms of biomarkers, quality control of glycoprotein drugs, and the development of new drugs. However, usual approach such as proteoglycomics, glycoproteomics and glycomics which characterizes and/or identifies sugar chains, provides some structural information, but it does not provide any information of functionality of sugar chains. Therefore, in order to elucidate the function of glycans, functional glycomics which identifies the target glycoproteins and characterizes functional roles of sugar chains represents a promising approach. In this review, we show examples of functional glycomics technique using α 1,6 fucosyltransferase gene (Fut8) in order to identify the target glycoprotein(s). This approach is based on glycan profiling by CE/MS and LC/MS followed by proteomic approaches, including 2-DE/1-DE and lectin blot techniques and identification of functional changes of sugar chains.

Original languageEnglish
Pages (from-to)1881-1889
Number of pages9
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1764
Issue number12
DOIs
Publication statusPublished - Dec 2006

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Keywords

  • Core fucosylation
  • Functional glycomics
  • Knock down
  • Knock out
  • Lectin
  • MS
  • RNAi

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