Functional analyses reveal an important role for tyrosine residues in the staphylococcal multidrug efflux protein QacA

Jingqin Wu, Karl A. Hassan, Ronald A. Skurray, Melissa H. Brown

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background. The staphylococcal QacA multidrug efflux protein confers resistance to an exceptional number of structurally unrelated antimicrobial compounds. Aromatic amino acid residues have been shown to be highly important for the transport function of several multidrug transporters and are intimately involved in multidrug binding. This study investigated the structural and functional importance of the seven tyrosine residues in QacA by examining the phenotypic effect of incorporating conservative (aromatic) and non-conservative (non-aromatic) substitutions for these residues. Results. Determination of the resistance profiles and analysis of drug transport assays revealed that non-conservative substitutions for most tyrosine residues influenced the QacA drug recognition spectrum. However, an aromatic residue at three tyrosine positions, 63, 410 and 429, was of importance for QacA-mediated transport and resistance to the majority of substrates tested. Conclusion. A tyrosine or phenylalanine residue at amino acid positions corresponding to 63 of QacA in related drug efflux proteins is found to be highly conserved. Therefore, an aromatic side chain at this position is likely to partake in a function common to these drug transporters, such as proton translocation or essential intramolecular contacts, whereas aromatic residues at the non-conserved 410 and 429 positions are expected to mediate a QacA-specific function, possibly forming or stabilising part of the QacA drug binding region.

LanguageEnglish
Article number147
Pages1-7
Number of pages7
JournalBMC Microbiology
Volume8
DOIs
Publication statusPublished - 2008

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Tyrosine
Pharmaceutical Preparations
Proteins
Aromatic Amino Acids
Phenylalanine
Protons
Amino Acids

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title = "Functional analyses reveal an important role for tyrosine residues in the staphylococcal multidrug efflux protein QacA",
abstract = "Background. The staphylococcal QacA multidrug efflux protein confers resistance to an exceptional number of structurally unrelated antimicrobial compounds. Aromatic amino acid residues have been shown to be highly important for the transport function of several multidrug transporters and are intimately involved in multidrug binding. This study investigated the structural and functional importance of the seven tyrosine residues in QacA by examining the phenotypic effect of incorporating conservative (aromatic) and non-conservative (non-aromatic) substitutions for these residues. Results. Determination of the resistance profiles and analysis of drug transport assays revealed that non-conservative substitutions for most tyrosine residues influenced the QacA drug recognition spectrum. However, an aromatic residue at three tyrosine positions, 63, 410 and 429, was of importance for QacA-mediated transport and resistance to the majority of substrates tested. Conclusion. A tyrosine or phenylalanine residue at amino acid positions corresponding to 63 of QacA in related drug efflux proteins is found to be highly conserved. Therefore, an aromatic side chain at this position is likely to partake in a function common to these drug transporters, such as proton translocation or essential intramolecular contacts, whereas aromatic residues at the non-conserved 410 and 429 positions are expected to mediate a QacA-specific function, possibly forming or stabilising part of the QacA drug binding region.",
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Functional analyses reveal an important role for tyrosine residues in the staphylococcal multidrug efflux protein QacA. / Wu, Jingqin; Hassan, Karl A.; Skurray, Ronald A.; Brown, Melissa H.

In: BMC Microbiology, Vol. 8, 147, 2008, p. 1-7.

Research output: Contribution to journalArticleResearchpeer-review

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