Functionality and stability of heparin immobilized onto poly(dimethylsiloxane)

Sara Thorslund, Javier Sanchez, Rolf Larsson, Fredrik Nikolajeff, Jonas Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Poly(dimethylsiloxane) (PDMS) has become an attractive material when working in the field of microfluidics, mainly because of the rapid prototyping process it involves. The increased surface volume ratio in microchannels makes the interaction between sample and material surface highly important, evident when handling complex biological samples such as plasma or blood. This study demonstrates a new grade of non-covalent heparin surface that adds efficient anticoagulant property to the PDMS material. The surface modification is a simple and fast one-step process performed at neutral pH, optimal when working with closed microsystems. The heparin formed a uniform and functional coating on hydrophobic PDMS with comparatively high level of antithrombin-binding capacity. In addition, long-term studies revelaed that the immobilized heparin was more or less stable in the microchannels over a time of three weeks. Recalcified plasma in contact with native PDMS showed complete coagulation after 1 h, while no fibrin formation was detected in plasma incubated on heparin-coated PDMS within the same time. In conclusion, we see the heparin coating developed and evaluated in this study as a tool that greatly facilitates the use of PDMS in microfluidics dealing with plasma or blood samples.

Original languageEnglish
Pages (from-to)76-81
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume45
Issue number2
DOIs
Publication statusPublished - 10 Oct 2005

Keywords

  • Hemocompatibility
  • Heparin
  • PDMS
  • Poly(dimethylsiloxane)
  • Stability

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