TY - JOUR
T1 - Functionalized single-walled carbon nanohorns for electrochemical biosensing
AU - Liu, Xiaoqing
AU - Li, Haijuan
AU - Wang, Fuan
AU - Zhu, Shuyun
AU - Wang, Yuling
AU - Xu, Guobao
PY - 2010/6
Y1 - 2010/6
N2 - Single-walled carbon nanohorns (SWNHs), distinguished by their high purity and distinct structure, were noncovalently functionalized with poly(sodium 4-styrenesulfonate). The functionalized SWNHs were characterized by scanning electron microscopy, atomic force microscopy, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, and thermogravimetry. Heme protein myoglobin was adsorbed onto surface of functionalized SWNHs to prepare electrochemical biosensor. Surface assembly process and direct electrochemistry of immobilized myoglobin were investigated by electrochemical impedance spectroscopy and cyclic voltammetry, respectively. The proposed biosensor exhibited good electrocatalysis to the reduction of hydrogen peroxide. The response was linear over the range 3-350μM with a detection limit of 0.5μM. Good reproducibility and stability of the biosensor were obtained toward hydrogen peroxide detection.
AB - Single-walled carbon nanohorns (SWNHs), distinguished by their high purity and distinct structure, were noncovalently functionalized with poly(sodium 4-styrenesulfonate). The functionalized SWNHs were characterized by scanning electron microscopy, atomic force microscopy, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, and thermogravimetry. Heme protein myoglobin was adsorbed onto surface of functionalized SWNHs to prepare electrochemical biosensor. Surface assembly process and direct electrochemistry of immobilized myoglobin were investigated by electrochemical impedance spectroscopy and cyclic voltammetry, respectively. The proposed biosensor exhibited good electrocatalysis to the reduction of hydrogen peroxide. The response was linear over the range 3-350μM with a detection limit of 0.5μM. Good reproducibility and stability of the biosensor were obtained toward hydrogen peroxide detection.
KW - Biosensors
KW - Carbon nanohorns
KW - Carbon nanotubes
KW - Heme proteins
KW - Noncovalent functionalization
UR - http://www.scopus.com/inward/record.url?scp=77952582883&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2010.02.027
DO - 10.1016/j.bios.2010.02.027
M3 - Article
C2 - 20299202
AN - SCOPUS:77952582883
SN - 0956-5663
VL - 25
SP - 2194
EP - 2199
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 10
ER -