Fungal proteins with mannanase activity identified directly from a Congo Red stained zymogram by mass spectrometry

Robyn Peterson*, Jasmine Grinyer, Janice Joss, Alamgir Khan, Helena Nevalainen

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Secreted fungal proteins with mannanase activity were identified by mass spectrometry of bands excised from a Congo Red stained zymogram containing locust bean gum as substrate. This technique circumvents the need to locate corresponding bands on a parallel gel without substrate and provides good accuracy in targeting proteins for identification.

Original languageEnglish
Pages (from-to)374-377
Number of pages4
JournalJournal of Microbiological Methods
Volume79
Issue number3
DOIs
Publication statusPublished - Dec 2009

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