Fungal proteins with mannanase activity identified directly from a Congo Red stained zymogram by mass spectrometry

Robyn Peterson; Jasmine Grinyer; Janice Joss; Alamgir Khan; Helena Nevalainen

doi:10.1016/j.mimet.2009.10.009

Fungal proteins with mannanase activity identified directly from a Congo Red stained zymogram by mass spectrometry

Robyn Peterson^*, Jasmine Grinyer, Janice Joss, Alamgir Khan, Helena Nevalainen

^*Corresponding author for this work

Faculty of Science and Engineering

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Secreted fungal proteins with mannanase activity were identified by mass spectrometry of bands excised from a Congo Red stained zymogram containing locust bean gum as substrate. This technique circumvents the need to locate corresponding bands on a parallel gel without substrate and provides good accuracy in targeting proteins for identification.

Original language	English
Pages (from-to)	374-377
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	79
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2009.10.009
Publication status	Published - Dec 2009

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10.1016/j.mimet.2009.10.009

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title = "Fungal proteins with mannanase activity identified directly from a Congo Red stained zymogram by mass spectrometry",

abstract = "Secreted fungal proteins with mannanase activity were identified by mass spectrometry of bands excised from a Congo Red stained zymogram containing locust bean gum as substrate. This technique circumvents the need to locate corresponding bands on a parallel gel without substrate and provides good accuracy in targeting proteins for identification.",

author = "Robyn Peterson and Jasmine Grinyer and Janice Joss and Alamgir Khan and Helena Nevalainen",

year = "2009",

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doi = "10.1016/j.mimet.2009.10.009",

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journal = "Journal of Microbiological Methods",

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T1 - Fungal proteins with mannanase activity identified directly from a Congo Red stained zymogram by mass spectrometry

AU - Peterson, Robyn

AU - Grinyer, Jasmine

AU - Joss, Janice

AU - Khan, Alamgir

AU - Nevalainen, Helena

PY - 2009/12

Y1 - 2009/12

N2 - Secreted fungal proteins with mannanase activity were identified by mass spectrometry of bands excised from a Congo Red stained zymogram containing locust bean gum as substrate. This technique circumvents the need to locate corresponding bands on a parallel gel without substrate and provides good accuracy in targeting proteins for identification.

AB - Secreted fungal proteins with mannanase activity were identified by mass spectrometry of bands excised from a Congo Red stained zymogram containing locust bean gum as substrate. This technique circumvents the need to locate corresponding bands on a parallel gel without substrate and provides good accuracy in targeting proteins for identification.

UR - https://www.scopus.com/pages/publications/70449522158

U2 - 10.1016/j.mimet.2009.10.009

DO - 10.1016/j.mimet.2009.10.009

M3 - Article

C2 - 19854225

AN - SCOPUS:70449522158

SN - 0167-7012

VL - 79

SP - 374

EP - 377

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

Fungal proteins with mannanase activity identified directly from a Congo Red stained zymogram by mass spectrometry

Abstract

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