Abstract
A method for gene shuffling to form a mutant or chimeric gene, the method conmprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.
Translated title of the contribution | Gene shuffling with degenerate oligonucleotide |
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Original language | Danish |
Patent number | DK1320630T |
IPC | C12N 15/ 11 A I,C12Q 1/68 |
Priority date | 29/08/01 |
Publication status | Published - 8 Mar 2010 |