Gen-shuffling med degenereret oligonukleotid

Translated title of the contribution: Gene shuffling with degenerate oligonucleotide

Peter Leonard Bergquist (Inventor), Kaisu Milja Helena Nevalainen (Inventor), Moreland David Gibbs (Inventor)

Research output: Patent

Abstract

A method for gene shuffling to form a mutant or chimeric gene, the method conmprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.
Translated title of the contributionGene shuffling with degenerate oligonucleotide
Original languageDanish
Patent numberDK1320630T
IPCC12N 15/ 11 A I,C12Q 1/68
Priority date29/08/01
Publication statusPublished - 8 Mar 2010

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