Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming

Monique Bax; Rachelle Balez; Sonia Sanz Muñoz; Dzung Do-Ha; Claire H. Stevens; Tracey Berg; Mauricio C. Cabral-da-Silva; Martin Engel; Garth Nicholson; Shu Yang; Ian P. Blair; Lezanne Ooi

doi:10.1016/j.scr.2019.101530

Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNF^S621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming

Monique Bax, Rachelle Balez, Sonia Sanz Muñoz, Dzung Do-Ha, Claire H. Stevens, Tracey Berg, Mauricio C. Cabral-da-Silva, Martin Engel, Garth Nicholson, Shu Yang, Ian P. Blair, Lezanne Ooi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNF^S621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.

Original language	English
Article number	101530
Pages (from-to)	1-4
Number of pages	4
Journal	Stem Cell Research
Volume	40
DOIs	https://doi.org/10.1016/j.scr.2019.101530
Publication status	Published - 1 Oct 2019

Bibliographical note

Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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10.1016/j.scr.2019.101530

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Bax, M., Balez, R., Muñoz, S. S., Do-Ha, D., Stevens, C. H., Berg, T., Cabral-da-Silva, M. C., Engel, M., Nicholson, G., Yang, S., Blair, I. P., & Ooi, L. (2019). Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNF^S621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming. Stem Cell Research, 40, 1-4. [101530]. https://doi.org/10.1016/j.scr.2019.101530

Bax, Monique ; Balez, Rachelle ; Muñoz, Sonia Sanz ; Do-Ha, Dzung ; Stevens, Claire H. ; Berg, Tracey ; Cabral-da-Silva, Mauricio C. ; Engel, Martin ; Nicholson, Garth ; Yang, Shu ; Blair, Ian P. ; Ooi, Lezanne. / Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNF^S621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming. In: Stem Cell Research. 2019 ; Vol. 40. pp. 1-4.

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title = "Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming",

abstract = "Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.",

author = "Monique Bax and Rachelle Balez and Mu{\~n}oz, {Sonia Sanz} and Dzung Do-Ha and Stevens, {Claire H.} and Tracey Berg and Cabral-da-Silva, {Mauricio C.} and Martin Engel and Garth Nicholson and Shu Yang and Blair, {Ian P.} and Lezanne Ooi",

note = "Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",

year = "2019",

month = oct,

day = "1",

doi = "10.1016/j.scr.2019.101530",

language = "English",

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Bax, M, Balez, R, Muñoz, SS, Do-Ha, D, Stevens, CH, Berg, T, Cabral-da-Silva, MC, Engel, M, Nicholson, G, Yang, S , Blair, IP & Ooi, L 2019, 'Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNF^S621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming', Stem Cell Research, vol. 40, 101530, pp. 1-4. https://doi.org/10.1016/j.scr.2019.101530

Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNF^S621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming. / Bax, Monique; Balez, Rachelle; Muñoz, Sonia Sanz; Do-Ha, Dzung; Stevens, Claire H.; Berg, Tracey; Cabral-da-Silva, Mauricio C.; Engel, Martin; Nicholson, Garth; Yang, Shu ; Blair, Ian P.; Ooi, Lezanne.

In: Stem Cell Research, Vol. 40, 101530, 01.10.2019, p. 1-4.

Research output: Contribution to journal › Article › peer-review

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AU - Nicholson, Garth

AU - Yang, Shu

AU - Blair, Ian P.

AU - Ooi, Lezanne

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