Abstract
Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.
| Language | English |
|---|---|
| Article number | 101530 |
| Pages | 1-4 |
| Number of pages | 4 |
| Journal | Stem Cell Research |
| Volume | 40 |
| DOIs | |
| Publication status | Published - 1 Oct 2019 |
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Bibliographical note
Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.Cite this
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Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming. / Bax, Monique; Balez, Rachelle; Muñoz, Sonia Sanz; Do-Ha, Dzung; Stevens, Claire H.; Berg, Tracey; Cabral-da-Silva, Mauricio C.; Engel, Martin; Nicholson, Garth; Yang, Shu; Blair, Ian P.; Ooi, Lezanne.
In: Stem Cell Research, Vol. 40, 101530, 01.10.2019, p. 1-4.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming
AU - Bax, Monique
AU - Balez, Rachelle
AU - Muñoz, Sonia Sanz
AU - Do-Ha, Dzung
AU - Stevens, Claire H.
AU - Berg, Tracey
AU - Cabral-da-Silva, Mauricio C.
AU - Engel, Martin
AU - Nicholson, Garth
AU - Yang, Shu
AU - Blair, Ian P.
AU - Ooi, Lezanne
N1 - Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.
AB - Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.
UR - http://www.scopus.com/inward/record.url?scp=85070890584&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2019.101530
DO - 10.1016/j.scr.2019.101530
M3 - Article
VL - 40
SP - 1
EP - 4
JO - Stem Cell Research
T2 - Stem Cell Research
JF - Stem Cell Research
SN - 1873-5061
M1 - 101530
ER -