Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming

Monique Bax, Rachelle Balez, Sonia Sanz Muñoz, Dzung Do-Ha, Claire H. Stevens, Tracey Berg, Mauricio C. Cabral-da-Silva, Martin Engel, Garth Nicholson, Shu Yang, Ian P. Blair, Lezanne Ooi*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)
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    Abstract

    Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.

    Original languageEnglish
    Article number101530
    Pages (from-to)1-4
    Number of pages4
    JournalStem Cell Research
    Volume40
    DOIs
    Publication statusPublished - 1 Oct 2019

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    Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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