Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming

Monique Bax, Rachelle Balez, Sonia Sanz Muñoz, Dzung Do-Ha, Claire H. Stevens, Tracey Berg, Mauricio C. Cabral-da-Silva, Martin Engel, Garth Nicholson, Shu Yang, Ian P. Blair, Lezanne Ooi

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.

LanguageEnglish
Article number101530
Pages1-4
Number of pages4
JournalStem Cell Research
Volume40
DOIs
Publication statusPublished - 1 Oct 2019

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Induced Pluripotent Stem Cells
Cyclins
Amyotrophic Lateral Sclerosis
Fibroblasts
Cell Line
Germ Layers
Messenger RNA
Skin
Mutation
MicroRNAs
Genes
Amyotrophic lateral sclerosis 1
Cellular Reprogramming

Bibliographical note

Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Cite this

Bax, Monique ; Balez, Rachelle ; Muñoz, Sonia Sanz ; Do-Ha, Dzung ; Stevens, Claire H. ; Berg, Tracey ; Cabral-da-Silva, Mauricio C. ; Engel, Martin ; Nicholson, Garth ; Yang, Shu ; Blair, Ian P. ; Ooi, Lezanne. / Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming. In: Stem Cell Research. 2019 ; Vol. 40. pp. 1-4.
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abstract = "Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.",
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Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming. / Bax, Monique; Balez, Rachelle; Muñoz, Sonia Sanz; Do-Ha, Dzung; Stevens, Claire H.; Berg, Tracey; Cabral-da-Silva, Mauricio C.; Engel, Martin; Nicholson, Garth; Yang, Shu; Blair, Ian P.; Ooi, Lezanne.

In: Stem Cell Research, Vol. 40, 101530, 01.10.2019, p. 1-4.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Blair, Ian P.

AU - Ooi, Lezanne

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