TY - JOUR
T1 - Generation of a cre recombinase-conditional Nos1ap over-expression transgenic mouse
AU - Auer, Dallas R.
AU - Sysa-Shah, Polina
AU - Bedja, Djahida
AU - Simmers, Jessica L.
AU - Pak, Evgenia
AU - Dutra, Amalia
AU - Cohn, Ronald
AU - Gabrielson, Kathleen L.
AU - Chakravarti, Aravinda
AU - Kapoor, Ashish
PY - 2014/6
Y1 - 2014/6
N2 - Polymorphic non-coding variants at the NOS1AP locus have been associated with the common cardiac, metabolic and neurological traits and diseases. Although, in vitro gene targeting-based cellular and biochemical studies have shed some light on NOS1AP function in cardiac and neuronal tissue, to enhance our understanding of NOS1AP function in mammalian physiology and disease, we report the generation of cre recombinase-conditional Nos1ap over-expression transgenic mice (Nos1ap Tg). Conditional transgenic mice were generated by the pronuclear injection method and three independent, single-site, multiple copies integration event-based founder lines were selected. For heart-restricted over-expression, Nos1ap Tg mice were crossed with Mlc2v-cre and Nos1ap transcript over-expression was observed in left ventricles from Nos1ap Tg; Mlc2v-cre F1 mice. We believe that with the potential of conditional over-expression, Nos1ap Tg mice will be a useful resource in studying NOS1AP function in various tissues under physiological and disease states.
AB - Polymorphic non-coding variants at the NOS1AP locus have been associated with the common cardiac, metabolic and neurological traits and diseases. Although, in vitro gene targeting-based cellular and biochemical studies have shed some light on NOS1AP function in cardiac and neuronal tissue, to enhance our understanding of NOS1AP function in mammalian physiology and disease, we report the generation of cre recombinase-conditional Nos1ap over-expression transgenic mice (Nos1ap Tg). Conditional transgenic mice were generated by the pronuclear injection method and three independent, single-site, multiple copies integration event-based founder lines were selected. For heart-restricted over-expression, Nos1ap Tg mice were crossed with Mlc2v-cre and Nos1ap transcript over-expression was observed in left ventricles from Nos1ap Tg; Mlc2v-cre F1 mice. We believe that with the potential of conditional over-expression, Nos1ap Tg mice will be a useful resource in studying NOS1AP function in various tissues under physiological and disease states.
KW - Cardiac arrhythmia
KW - Conditional over-expression
KW - GENOME-wide association studies
KW - NOS1AP
KW - QT interval
KW - Sudden cardiac death
KW - Transgenic mouse
UR - http://www.scopus.com/inward/record.url?scp=84899911713&partnerID=8YFLogxK
U2 - 10.1007/s10529-014-1473-x
DO - 10.1007/s10529-014-1473-x
M3 - Article
C2 - 24563304
AN - SCOPUS:84899911713
SN - 0141-5492
VL - 36
SP - 1179
EP - 1185
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 6
ER -