Generation of a new tau knockout (tau Δex1) line using CRISPR/Cas9 genome editing in mice

Daniel C. S. Tan, Sherilyn Yao, Arne Ittner, Josefine Bertz, Yazi D. Ke, Lars M. Ittner*, Fabien Delerue

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Alzheimer's disease and other dementias present with tau pathology. Several mouse lines with knockout of the tau-encoding Mapt gene have been reported, yet findings often differed between lines and sites. Here, we report a new tau knockout strain (tau Δex1), generated by CRISPR/Cas9-mediated genome editing of intron -1/exon 1 of Mapt in C57Bl/6J mice. Tau Δex1 mice had no overt phenotype, but, in line with previous models, they showed a significantly reduced susceptibility to excitotoxic seizures, with normal memory formation in young mice. This new in vivo resource will be made freely available to the research community.

Original languageEnglish
Pages (from-to)571-578
Number of pages8
JournalJournal of Alzheimer's Disease
Volume62
Issue number2
DOIs
Publication statusPublished - 1 Jan 2018
Externally publishedYes

Keywords

  • C57Bl/6
  • CRISPR
  • gene knockout
  • mouse
  • tau

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