Generation of a sexually mature individual of the Eastern dwarf tree frog, Litoria fallax, from cryopreserved testicular macerates: proof of capacity of cryopreserved sperm derived offspring to complete development

Rose Upton, Simon Clulow, Michael Mahony, John Clulow

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)
    11 Downloads (Pure)

    Abstract

    Amphibians are the most threatened vertebrate class globally based on recent rates of decline and extinction. Sperm cryopreservation and other assisted reproductive technologies have the potential to help manage small and threatened populations and prevent extinctions. There are a growing number of reports of recovery of amphibian sperm after cryopreservation, but relatively few published reports of amphibian embryos generated from frozen sperm developing beyond metamorphosis to the adult stage and achieving sexual maturation. In this study on the Eastern dwarf tree frog (Litoria fallax), a temperate amphibian species from eastern Australia, a small number of viable metamorphs and one sexually mature male frog (itself producing sperm) were produced from cryopreserved sperm, demonstrating the capacity of embryos generated from cryopreserved sperm to complete the life cycle to sexual maturity. Low progression rates between developmental stages were not deemed to be due to effects of cryopreservation, since control embryos from unfrozen sperm had a similarly low progression rate through development.
    Original languageEnglish
    Article numbercoy043
    Pages (from-to)1-5
    Number of pages5
    JournalConservation Physiology
    Volume6
    Issue number1
    DOIs
    Publication statusPublished - 15 Aug 2018

    Bibliographical note

    Copyright the Author(s) 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

    Keywords

    • ART
    • assisted reproductive technologies
    • conservation
    • cryopreservation
    • IVF
    • sperm

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