Abstract
The use of genetically modified mice has significantly contributed to studies on both physiological and pathological in vivo processes. The pronuclear injection of DNA expression constructs into fertilized oocytes remains the most commonly used technique to generate transgenic mice for overexpression. With the introduction of CRISPR technology for gene targeting, pronuclear injection into fertilized oocytes has been extended to the generation of both knockout and knockin mice. This work describes the preparation of DNA for injection and the generation of CRISPR guides for gene targeting, with a particular emphasis on quality control. The genotyping procedures required for the identification of potential founders are critical. Innovative genotyping strategies that take advantage of the “multiplexing” capabilities of CRISPR are presented herein. Surgical procedures are also outlined. Together, the steps of the protocol will allow for the generation of genetically modified mice and for the subsequent establishment of mouse colonies for a plethora of research fields, including immunology, neuroscience, cancer, physiology, development, and others.
Original language | English |
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Article number | e55765 |
Pages (from-to) | 1-12 |
Number of pages | 12 |
Journal | Journal of Visualized Experiments |
Volume | 2017 |
Issue number | 124 |
DOIs | |
Publication status | Published - 15 Jun 2017 |
Externally published | Yes |
Keywords
- CRISPR
- Cytoplasmic
- Developmental Biology
- Genome editing
- Issue 124
- Mice
- Microinjection
- Oocytes
- Pronuclear
- Transgenic