Genes for phaseolotoxin synthesis are located on the chromosome of Pseudomonas syringae pv. phaseolicola

Neil B. Quigley, David Lane, Peter L. Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Transposon mutagenesis was used to locate the genes for phaseolotoxin biosynthesis in Pseudomonas syringae pathovar. phaseolicola. Mutants unable to produce toxin were obtained that carried Tn5 on different chromosomal restriction fragments. None of the Tn5-induced nontoxigenic mutants carried the transposon in plasmid DNA. The insertion of Tn5 into tox DNA was confirmed by site-directed mutagenesis. The results reported here suggest the involvement of at least five chromosomal genes in phaseolotoxin biosynthesis. All of the toxinminus mutants retained both full pathogenicity on beans and resistance to the toxin.

Original languageEnglish
Pages (from-to)295-299
Number of pages5
JournalCurrent Microbiology
Volume12
Issue number5
DOIs
Publication statusPublished - Sep 1985
Externally publishedYes

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