Genetic characterisation of Cryptosporidium from a wild population of eastern grey kangaroos Macropus giganteus inhabiting a water catchment

Michelle L. Power*, Martin B. Slade, Nicholas C. Sangster, Duncan A. Veal

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    44 Citations (Scopus)

    Abstract

    Molecular characterisation of Cryptosporidium oocysts isolated from faeces collected from eastern grey kangaroos Macropus giganteus inhabiting an Australian water catchment revealed that this host was susceptible to three types of Cryptosporidium. Nucleotide sequence analysis of the 18S rDNA, Cryptosporidium oocyst wall protein (COWP) and a 70 kDa heat shock protein (HSP70) identified an isolate identical to the described Cryptosporidium 'marsupial' genotype. A second isolate had less than 0.5% variation, compared to the described Cryptosporidium 'marsupial' genotype, within the sequences of the 18S rDNA, COWP and HSP70 and 10% variation in the internal transcribed spacer 1 (ITS1). Multilocus analysis of the third Cryptosporidium revealed a novel genotype that had a degree of genetic variation, at the four loci characterised, which was greater than or equivalent to that used to discriminate between currently recognised C ryptosporidium species. These findings have increased our current understanding on the molecular epidemiology of Cryptosporidium in Australian wildlife and have provided information on the types of Cryptosporidium marsupials may shed into the environment.

    Original languageEnglish
    Pages (from-to)59-67
    Number of pages9
    JournalInfection, Genetics and Evolution
    Volume4
    Issue number1
    DOIs
    Publication statusPublished - Mar 2004

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