Genetic variation and post-translational modifications of Cytochrome c oxidase-1 (COX1) in different strains of Sordaria fimicola

Rabia Arif*, Syeda Hina Bukhari, Muhammad Ishfaq, Memuna Ghafoor Shahid, Siu Fai Lee, Muhammad Saleem

*Corresponding author for this work

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    Abstract

    Post translational modifications, not only alter the protein structure but also its dynamics due to which there is a definite change in the performance of that protein resulting in remarkable diversity and complexity. The main purpose behind this study was to point out genetic variations in Cytochrome c oxidase-1 at gene level, protein level and modifications of protein in six parental strains and subsequent generations (F3 and F4) of Sordaria fimicola. All genetic variations and protein modifications were compared with reference organism (Neurospora crassa). Overall, point mutations on 10 different positions were observed in all strains when compared with reference organism, 7 of these were exhibited by the strains isolated from harsh environmental conditions while variations on 3 positions were common between strains of two opposite environments. Various post-translational modification predictor tools were used to predict post-translational modifications. The accuracy of results of PTMs can be enhanced by combining some bioinformatics techniques with mass spectrophotometry, which fascinate the researchers who are working in the field of proteomics.

    Original languageEnglish
    Pages (from-to)1055-1062
    Number of pages8
    JournalInternational Journal of Agriculture and Biology
    Volume21
    Issue number5
    DOIs
    Publication statusPublished - 2019

    Bibliographical note

    Copyright the Publisher 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

    Keywords

    • Evolution canyon
    • Glycosylation
    • Nucleotide
    • Polymorphisms
    • Phosphorylation

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