Projects per year
Activation of a cryptic polyketide synthase gene cluster hkn from Aspergillus hancockii via overexpression of the gene-cluster-specific transcription factor HknR led to the discovery of a novel polycyclic metabolite, which we named hancockinone A. The compound features an unprecedented prenylated 6/6/6/5 tetracarbocyclic skeleton and shows moderate antibacterial activity. Heterologous expression, substrate feeding, and in vitro assays confirmed the role of cytochrome P450 HknE in constructing the five-membered ring in hancockinone A from the precursor neosartoricin B.
|Number of pages||5|
|Publication status||Published - 19 Nov 2021|
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UWA led: CRISPR-based pathway activation for bioactive molecule discovery in fungi
Chooi, Y. H., Piggott, A. & Keller, N.
11/02/21 → 10/02/24
BioAustralis, towards the future
Lacey, E., Piggott, A., Chooi, Y. H. & Page, S.
19/10/18 → 31/12/21