Genome of the human hookworm Necator americanus

Yat T. Tang, Xin Gao, Bruce A. Rosa, Sahar Abubucker, Kymberlie Hallsworth-Pepin, John Martin, Rahul Tyagi, Esley Heizer, Xu Zhang, Veena Bhonagiri-Palsikar, Patrick Minx, Wesley C. Warren, Qi Wang, Bin Zhan, Peter J. Hotez, Paul W. Sternberg, Annette Dougall, Soraya Torres Gaze, Jason Mulvenna, Javier Sotillo & 9 others Shoba Ranganathan, Elida M. Rabelo, Richard K. Wilson, Philip L. Felgner, Jeffrey Bethony, John M. Hawdon, Robin B. Gasser, Alex Loukas, Makedonka Mitreva

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The hookworm Necator americanus is the predominant soil-transmitted human parasite. Adult worms feed on blood in the small intestine, causing iron-deficiency anemia, malnutrition, growth and development stunting in children, and severe morbidity and mortality during pregnancy in women. We report sequencing and assembly of the N. americanus genome (244 Mb, 19,151 genes). Characterization of this first hookworm genome sequence identified genes orchestrating the hookworm's invasion of the human host, genes involved in blood feeding and development, and genes encoding proteins that represent new potential drug targets against hookworms. N. americanus has undergone a considerable and unique expansion of immunomodulator proteins, some of which we highlight as potential treatments against inflammatory diseases. We also used a protein microarray to demonstrate a postgenomic application of the hookworm genome sequence. This genome provides an invaluable resource to boost ongoing efforts toward fundamental and applied postgenomic research, including the development of new methods to control hookworm and human immunological diseases.

LanguageEnglish
Pages261-269
Number of pages9
JournalNature Genetics
Volume46
Issue number3
DOIs
Publication statusPublished - 2014

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Necator americanus
Ancylostomatoidea
Human Genome
Genome
Growth Disorders
Genes
Protein Array Analysis
Iron-Deficiency Anemias
Immune System Diseases
Immunologic Factors
Growth and Development
Malnutrition
Small Intestine
Parasites
Proteins
Soil
Morbidity
Pregnancy
Mortality
Research

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Tang, Y. T., Gao, X., Rosa, B. A., Abubucker, S., Hallsworth-Pepin, K., Martin, J., ... Mitreva, M. (2014). Genome of the human hookworm Necator americanus. Nature Genetics, 46(3), 261-269. https://doi.org/10.1038/ng.2875
Tang, Yat T. ; Gao, Xin ; Rosa, Bruce A. ; Abubucker, Sahar ; Hallsworth-Pepin, Kymberlie ; Martin, John ; Tyagi, Rahul ; Heizer, Esley ; Zhang, Xu ; Bhonagiri-Palsikar, Veena ; Minx, Patrick ; Warren, Wesley C. ; Wang, Qi ; Zhan, Bin ; Hotez, Peter J. ; Sternberg, Paul W. ; Dougall, Annette ; Gaze, Soraya Torres ; Mulvenna, Jason ; Sotillo, Javier ; Ranganathan, Shoba ; Rabelo, Elida M. ; Wilson, Richard K. ; Felgner, Philip L. ; Bethony, Jeffrey ; Hawdon, John M. ; Gasser, Robin B. ; Loukas, Alex ; Mitreva, Makedonka. / Genome of the human hookworm Necator americanus. In: Nature Genetics. 2014 ; Vol. 46, No. 3. pp. 261-269.
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abstract = "The hookworm Necator americanus is the predominant soil-transmitted human parasite. Adult worms feed on blood in the small intestine, causing iron-deficiency anemia, malnutrition, growth and development stunting in children, and severe morbidity and mortality during pregnancy in women. We report sequencing and assembly of the N. americanus genome (244 Mb, 19,151 genes). Characterization of this first hookworm genome sequence identified genes orchestrating the hookworm's invasion of the human host, genes involved in blood feeding and development, and genes encoding proteins that represent new potential drug targets against hookworms. N. americanus has undergone a considerable and unique expansion of immunomodulator proteins, some of which we highlight as potential treatments against inflammatory diseases. We also used a protein microarray to demonstrate a postgenomic application of the hookworm genome sequence. This genome provides an invaluable resource to boost ongoing efforts toward fundamental and applied postgenomic research, including the development of new methods to control hookworm and human immunological diseases.",
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Tang, YT, Gao, X, Rosa, BA, Abubucker, S, Hallsworth-Pepin, K, Martin, J, Tyagi, R, Heizer, E, Zhang, X, Bhonagiri-Palsikar, V, Minx, P, Warren, WC, Wang, Q, Zhan, B, Hotez, PJ, Sternberg, PW, Dougall, A, Gaze, ST, Mulvenna, J, Sotillo, J, Ranganathan, S, Rabelo, EM, Wilson, RK, Felgner, PL, Bethony, J, Hawdon, JM, Gasser, RB, Loukas, A & Mitreva, M 2014, 'Genome of the human hookworm Necator americanus', Nature Genetics, vol. 46, no. 3, pp. 261-269. https://doi.org/10.1038/ng.2875

Genome of the human hookworm Necator americanus. / Tang, Yat T.; Gao, Xin; Rosa, Bruce A.; Abubucker, Sahar; Hallsworth-Pepin, Kymberlie; Martin, John; Tyagi, Rahul; Heizer, Esley; Zhang, Xu; Bhonagiri-Palsikar, Veena; Minx, Patrick; Warren, Wesley C.; Wang, Qi; Zhan, Bin; Hotez, Peter J.; Sternberg, Paul W.; Dougall, Annette; Gaze, Soraya Torres; Mulvenna, Jason; Sotillo, Javier; Ranganathan, Shoba; Rabelo, Elida M.; Wilson, Richard K.; Felgner, Philip L.; Bethony, Jeffrey; Hawdon, John M.; Gasser, Robin B.; Loukas, Alex; Mitreva, Makedonka.

In: Nature Genetics, Vol. 46, No. 3, 2014, p. 261-269.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Martin, John

AU - Tyagi, Rahul

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Tang YT, Gao X, Rosa BA, Abubucker S, Hallsworth-Pepin K, Martin J et al. Genome of the human hookworm Necator americanus. Nature Genetics. 2014;46(3):261-269. https://doi.org/10.1038/ng.2875