Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus

Garry S A Myers; Dane Parker; Keith Al-Hasani; Ruth M. Kennan; Torsten Seemann; Qinghu Ren; Jonathan H. Badger; Jeremy D. Selengut; Robert T. DeBoy; Hervé Tettelin; John D. Boyce; Victoria P. McCarl; Xiaoyan Han; William C. Nelson; Ramana Madupu; Yasmin Mohamoud; Tara Holley; Nadia Fedorova; Hoda Khouri; Steven P. Bottomley; Richard J. Whittington; Ben Adler; J. Glenn Songer; Julian I. Rood; Ian T. Paulsen

doi:10.1038/nbt1302

Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus

Garry S A Myers, Dane Parker, Keith Al-Hasani, Ruth M. Kennan, Torsten Seemann, Qinghu Ren, Jonathan H. Badger, Jeremy D. Selengut, Robert T. DeBoy, Hervé Tettelin, John D. Boyce, Victoria P. McCarl, Xiaoyan Han, William C. Nelson, Ramana Madupu, Yasmin Mohamoud, Tara Holley, Nadia Fedorova, Hoda Khouri, Steven P. BottomleyRichard J. Whittington, Ben Adler, J. Glenn Songer, Julian I. Rood, Ian T. Paulsen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

61 Citations (Scopus)

Abstract

Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine.

Original language	English
Pages (from-to)	569-575
Number of pages	7
Journal	Nature Biotechnology
Volume	25
Issue number	5
DOIs	https://doi.org/10.1038/nbt1302
Publication status	Published - May 2007
Externally published	Yes

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Myers, G. S. A., Parker, D., Al-Hasani, K., Kennan, R. M., Seemann, T., Ren, Q., Badger, J. H., Selengut, J. D., DeBoy, R. T., Tettelin, H., Boyce, J. D., McCarl, V. P., Han, X., Nelson, W. C., Madupu, R., Mohamoud, Y., Holley, T., Fedorova, N., Khouri, H., ... Paulsen, I. T. (2007). Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus. Nature Biotechnology, 25(5), 569-575. https://doi.org/10.1038/nbt1302

@article{88964b0c494a4fe7ad497493ee476552,

title = "Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus",

abstract = "Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine.",

author = "Myers, {Garry S A} and Dane Parker and Keith Al-Hasani and Kennan, {Ruth M.} and Torsten Seemann and Qinghu Ren and Badger, {Jonathan H.} and Selengut, {Jeremy D.} and DeBoy, {Robert T.} and Herv{\'e} Tettelin and Boyce, {John D.} and McCarl, {Victoria P.} and Xiaoyan Han and Nelson, {William C.} and Ramana Madupu and Yasmin Mohamoud and Tara Holley and Nadia Fedorova and Hoda Khouri and Bottomley, {Steven P.} and Whittington, {Richard J.} and Ben Adler and Songer, {J. Glenn} and Rood, {Julian I.} and Paulsen, {Ian T.}",

year = "2007",

month = may,

doi = "10.1038/nbt1302",

language = "English",

volume = "25",

pages = "569--575",

journal = "Nature Biotechnology",

issn = "1087-0156",

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Myers, GSA, Parker, D, Al-Hasani, K, Kennan, RM, Seemann, T, Ren, Q, Badger, JH, Selengut, JD, DeBoy, RT, Tettelin, H, Boyce, JD, McCarl, VP, Han, X, Nelson, WC, Madupu, R, Mohamoud, Y, Holley, T, Fedorova, N, Khouri, H, Bottomley, SP, Whittington, RJ, Adler, B, Songer, JG, Rood, JI & Paulsen, IT 2007, 'Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus', Nature Biotechnology, vol. 25, no. 5, pp. 569-575. https://doi.org/10.1038/nbt1302

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AU - Myers, Garry S A

AU - Parker, Dane

AU - Al-Hasani, Keith

AU - Kennan, Ruth M.

AU - Seemann, Torsten

AU - Ren, Qinghu

AU - Badger, Jonathan H.

AU - Selengut, Jeremy D.

AU - DeBoy, Robert T.

AU - Tettelin, Hervé

AU - Boyce, John D.

AU - McCarl, Victoria P.

AU - Han, Xiaoyan

AU - Nelson, William C.

AU - Madupu, Ramana

AU - Mohamoud, Yasmin

AU - Holley, Tara

AU - Fedorova, Nadia

AU - Khouri, Hoda

AU - Bottomley, Steven P.

AU - Whittington, Richard J.

AU - Adler, Ben

AU - Songer, J. Glenn

AU - Rood, Julian I.

AU - Paulsen, Ian T.

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AB - Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine.

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VL - 25

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JO - Nature Biotechnology

JF - Nature Biotechnology

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ER -

Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus

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