Genome-wide transcription analysis of clinal genetic variation in drosophila

Ying Chen*, Siu F. Lee, Eric Blanc, Caroline Reuter, Bregje Wertheim, Pedro Martinez-Diaz, Ary A. Hoffmann, Linda Partridge

*Corresponding author for this work

Research output: Contribution to journalArticle

13 Citations (Scopus)
8 Downloads (Pure)

Abstract

Clinal variation in quantitative traits is widespread, but its genetic basis awaits identification. Drosophila melanogaster shows adaptive, clinal variation in traits such as body size along latitudinal gradients on multiple continents. To investigate genome wide transcription differentiation between North and South that might contribute to the clinal phenotypic variation, we compared RNA expression patterns during development of D. melanogaster from tropical northern and temperate southern populations using whole genome tiling arrays. We found that genes that were differentially expressed between the cline ends were generally associated with metabolism and growth, and experimental alteration of expression of a sample of them generally resulted in altered body size in the predicted direction, sometimes significantly so. We further identified the serpent (srp) transcription factor binding sites to be enriched near genes up-regulated in expression in the south. Analysis of clinal populations revealed a significant cline in the expression level of srp. Experimental over-expression of srp increased body size, as predicted from its clinal expression pattern, suggesting that it may be involved in regulating adaptive clinal variation in Drosophila. This study identified a handful of genes that contributed to clinal phenotypic variation through altered gene expression level, yet misexpression of individual gene led to modest body size change.

Original languageEnglish
Article numbere34620
Pages (from-to)1-8
Number of pages8
JournalPLoS ONE
Volume7
Issue number4
DOIs
Publication statusPublished - 13 Apr 2012
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2012. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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