Genomic fingerprinting and PCR analysis: rapid, sensitive and inexpensive means of differentiating strains of Pseudomonas-solanacearum

M GILLINGS, P FAHY

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

Abstract

Analysis of the total genomic restriction pattern, and restriction analysis of an amplified fragment of the endopolygalacturonase gene (pehH) both readily differentiate isolates, subdividing P. solanacearum into groups based on genetic relationships. They have allowed rapid identification of unknown isolates, and provided information on the evolution and epidemiology of individual strains. DNA fingerprinting has been used to establish the current distribution of individual strains, to reconstruct their possible origins and subsequent dissemination. In some cases, individual strains were shown to have restricted natural geographic distribution and-or plant hosts. The established classification schemes of biovars and races were confirmed by this analysis, with the exception of race 1, which cut across biovars and genetic groups.
Original languageEnglish
Title of host publicationBacterial wilt
EditorsGL Hartman, AC Hayward
PublisherAUSTRALIAN CENTRE INT AGRICULTURAL RESEARCH
Pages85-92
Number of pages8
ISBN (Print)1-86320-087-8, 9781863200875
Publication statusPublished - 1 Jan 1993
Externally publishedYes
Event2ND INTERNATIONAL CONF ON BACTERIAL WILT OF ALL CROPS - KAOHSIUNG
Duration: 28 Oct 199231 Oct 1992

Publication series

NameACIAR PROCEEDINGS SERIES
PublisherAUSTRALIAN CENTRE INT AGRICULTURAL RESEARCH
Volume45

Conference

Conference2ND INTERNATIONAL CONF ON BACTERIAL WILT OF ALL CROPS
CityKAOHSIUNG
Period28/10/9231/10/92

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