Genomic instability within centromeres of interspecific marsupial hybrids

Cushla J. Metcalfe, Kira V. Bulazel, Gianni C. Ferreri, Elizabeth Schroeder-Reiter, Gerhard Wanner, Willem Rens, Craig Obergfell, Mark D B Eldridge, Rachel J. O'Neill*

*Corresponding author for this work

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Several lines of evidence suggest that, within a lineage, particular genomic regions are subject to instability that can lead to specific types of chromosome rearrangements important in species incompatibility. Within family Macropodidae (kangaroos, wallabies, bettongs, and potoroos), which exhibit recent and extensive karyotypic evolution, rearrangements involve chiefly the centromere. We propose that centromeres are the primary target for destabilization in cases of genomic instability, such as interspecific hybridization, and participate in the formation of novel chromosome rearrangements. Here we use standard cytological staining, cross-species chromosome painting, DNA probe analyses, and scanning electron microscopy to examine four interspecific macropodid hybrids (Macropus rufogriseus X Macropus agilis). The parental complements share the same centric fusions relative to the presumed macropodid ancestral karyotype, but can be differentiated on the basis of heterochromatic content, M. rufogriseus having larger centromeres with large C-banding positive regions. All hybrids exhibited the same pattern of chromosomal instability and remodeling specifically within the centromeres derived from the maternal (M. rufogriseus) complement. This instability included amplification of a satellite repeat and a transposable element, changes in chromatin structure, and de novo whole-arm rearrangements. We discuss possible reasons and mechanisms for the centromeric instability and remodeling observed in all four macropodid hybrids.

Original languageEnglish
Pages (from-to)2507-2517
Number of pages11
JournalGenetics
Volume177
Issue number4
DOIs
Publication statusPublished - Dec 2007
Externally publishedYes

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