Global protein-level responses of halobacterium salinarum NRC-1 to prolonged changes in external sodium chloride concentrations

Stefan Leuko, Mark J. Raftery, Brendan P. Burns, Malcolm R. Walter, Brett A. Neilan

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Responses to changes in external salinity were examined in Halobacterium salinarum NRC-1. H. salinarum NRC-1 grows optimally at 4.3 M NaCl and is capable of growth between 2.6 and 5.1 M NaCl. Physiological changes following incubation at 2.6 M NaCl were investigated with respect to growth behavior and proteomic changes. Initial observations indicated delayed growth at low NaCl concentrations (2.6 M NaCl), and supplementation with different sugars, amino acids, or KCl to increase external osmotic pressure did not reverse these growth perturbations. To gain a more detailed insight into the adaptive responses of H. salinarum NRC-1 to changes in salinity, the proteome was characterized using iTRAQ (amine specific isobaric tagging reagents). Three hundred and nine differentially expressed proteins were shown to be associated with changes in the external sodium chloride concentration, with proteins associated with metabolism revealing the greatest response.

Original languageEnglish
Pages (from-to)2218-2225
Number of pages8
JournalJournal of Proteome Research
Volume8
Issue number5
DOIs
Publication statusPublished - 1 May 2009
Externally publishedYes

Keywords

  • Changed osmotic conditions
  • Halophilic Archaea
  • ITRAQ
  • Mass spectrometry
  • Proteomics

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