Global transcriptomic responses of Escherichia coli K-12 to volatile organic compounds

Pui Yi Yung, Letizia Lo Grasso, Abeed Fatima Mohidin, Enzo Acerbi, Jamie Hinks, Thomas Seviour, Enrico Marsili, Federico M. Lauro*

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Citations (Scopus)
4 Downloads (Pure)

Abstract

Volatile organic compounds (VOCs) are commonly used as solvents in various industrial settings. Many of them present a challenge to receiving environments, due to their toxicity and low bioavailability for degradation. Microorganisms are capable of sensing and responding to their surroundings and this makes them ideal detectors for toxic compounds. This study investigates the global transcriptomic responses of Escherichia coli K-12 to selected VOCs at sub-toxic levels. Cells grown in the presence of VOCs were harvested during exponential growth, followed by whole transcriptome shotgun sequencing (RNAseq). The analysis of the data revealed both shared and unique genetic responses compared to cells without exposure to VOCs. Results suggest that various functional gene categories, for example, those relating to Fe/S cluster biogenesis, oxidative stress responses and transport proteins, are responsive to selected VOCs in E. coli. The differential expression (DE) of genes was validated using GFP-promoter fusion assays. A variety of genes were differentially expressed even at non-inhibitory concentrations and when the cells are at their balanced-growth. Some of these genes belong to generic stress response and others could be specific to VOCs. Such candidate genes and their regulatory elements could be used as the basis for designing biosensors for selected VOCs.

Original languageEnglish
Article number19899
Pages (from-to)1-15
Number of pages15
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 28 Jan 2016
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2017. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Erratum can be found in Scientific Reports, Vol. 6, Article No. 19899. doi.org/10.1038/srep19899

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    Yung, P. Y., Grasso, L. L., Mohidin, A. F., Acerbi, E., Hinks, J., Seviour, T., ... Lauro, F. M. (2016). Global transcriptomic responses of Escherichia coli K-12 to volatile organic compounds. Scientific Reports, 6, 1-15. [19899]. https://doi.org/10.1038/srep19899