Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae

Simone Morris; Pradeep Manuneedhi Cholan; Warwick J. Britton; Stefan H Oehlers

doi:10.1038/s41598-021-98566-9

Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae

Simone Morris, Pradeep Manuneedhi Cholan^*, Warwick J. Britton, Stefan H Oehlers^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

63 Downloads (Pure)

Abstract

Hyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish larvae to model the effect of exogenous glucose on neutrophil and macrophage recruitment to a tail wound, wound-induced haemostasis, and chicken egg yolk feed challenge-induced hyperlipidaemia by supplementing larvae with exogenous glucose by immersion or injection. Neither method of glucose supplementation affected the recruitment of neutrophils and macrophages following tail transection. Glucose injection reduced thrombocyte retention and fibrin plug formation while only thrombocyte retention was reduced by glucose immersion following tail transection. We observed accelerated lipid accumulation in glucose-injected larvae challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose on inflammation, haemostasis, and hyperlipidaemia in zebrafish larvae compared to mammals.

Original language	English
Article number	19049
Pages (from-to)	1-8
Number of pages	8
Journal	Scientific Reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-98566-9
Publication status	Published - 24 Sept 2021
Externally published	Yes

Bibliographical note

Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Access to Document

10.1038/s41598-021-98566-9Licence: CC BY

Publisher version (open access)Final published version, 3.32 MBLicence: CC BY

Cite this

@article{1add32a711cd4d31a0fd076d89f1339c,

title = "Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae",

abstract = "Hyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish larvae to model the effect of exogenous glucose on neutrophil and macrophage recruitment to a tail wound, wound-induced haemostasis, and chicken egg yolk feed challenge-induced hyperlipidaemia by supplementing larvae with exogenous glucose by immersion or injection. Neither method of glucose supplementation affected the recruitment of neutrophils and macrophages following tail transection. Glucose injection reduced thrombocyte retention and fibrin plug formation while only thrombocyte retention was reduced by glucose immersion following tail transection. We observed accelerated lipid accumulation in glucose-injected larvae challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose on inflammation, haemostasis, and hyperlipidaemia in zebrafish larvae compared to mammals.",

author = "Simone Morris and Cholan, {Pradeep Manuneedhi} and Britton, {Warwick J.} and Oehlers, {Stefan H}",

note = "Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",

year = "2021",

month = sep,

day = "24",

doi = "10.1038/s41598-021-98566-9",

language = "English",

volume = "11",

pages = "1--8",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Springer, Springer Nature",

number = "1",

}

TY - JOUR

T1 - Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae

AU - Morris, Simone

AU - Cholan, Pradeep Manuneedhi

AU - Britton, Warwick J.

AU - Oehlers, Stefan H

N1 - Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2021/9/24

Y1 - 2021/9/24

N2 - Hyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish larvae to model the effect of exogenous glucose on neutrophil and macrophage recruitment to a tail wound, wound-induced haemostasis, and chicken egg yolk feed challenge-induced hyperlipidaemia by supplementing larvae with exogenous glucose by immersion or injection. Neither method of glucose supplementation affected the recruitment of neutrophils and macrophages following tail transection. Glucose injection reduced thrombocyte retention and fibrin plug formation while only thrombocyte retention was reduced by glucose immersion following tail transection. We observed accelerated lipid accumulation in glucose-injected larvae challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose on inflammation, haemostasis, and hyperlipidaemia in zebrafish larvae compared to mammals.

AB - Hyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish larvae to model the effect of exogenous glucose on neutrophil and macrophage recruitment to a tail wound, wound-induced haemostasis, and chicken egg yolk feed challenge-induced hyperlipidaemia by supplementing larvae with exogenous glucose by immersion or injection. Neither method of glucose supplementation affected the recruitment of neutrophils and macrophages following tail transection. Glucose injection reduced thrombocyte retention and fibrin plug formation while only thrombocyte retention was reduced by glucose immersion following tail transection. We observed accelerated lipid accumulation in glucose-injected larvae challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose on inflammation, haemostasis, and hyperlipidaemia in zebrafish larvae compared to mammals.

UR - https://purl.org/au-research/grants/nhmrc/GNT1153493

UR - http://www.scopus.com/inward/record.url?scp=85115728679&partnerID=8YFLogxK

U2 - 10.1038/s41598-021-98566-9

DO - 10.1038/s41598-021-98566-9

M3 - Article

C2 - 34561530

SN - 2045-2322

VL - 11

SP - 1

EP - 8

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 19049

ER -

Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this