Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae

Simone Morris, Pradeep Manuneedhi Cholan*, Warwick J. Britton, Stefan H Oehlers*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Hyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish larvae to model the effect of exogenous glucose on neutrophil and macrophage recruitment to a tail wound, wound-induced haemostasis, and chicken egg yolk feed challenge-induced hyperlipidaemia by supplementing larvae with exogenous glucose by immersion or injection. Neither method of glucose supplementation affected the recruitment of neutrophils and macrophages following tail transection. Glucose injection reduced thrombocyte retention and fibrin plug formation while only thrombocyte retention was reduced by glucose immersion following tail transection. We observed accelerated lipid accumulation in glucose-injected larvae challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose on inflammation, haemostasis, and hyperlipidaemia in zebrafish larvae compared to mammals.

Original languageEnglish
Article number19049
Pages (from-to)1-8
Number of pages8
JournalScientific Reports
Volume11
Issue number1
DOIs
Publication statusPublished - 24 Sep 2021
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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