Glycobiology and proteomics

is mass spectrometry the holy grail?

Nicolle H. Packer*, Mathew J. Harrison

*Corresponding author for this work

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

One characteristic of glycoproteins is that they are separated by two- dimensional electrophoresis (2-D PAGE) into typical 'trains' of protein spots which separate on the basis of different isoelectric point (pI) and/or molecular mass. The pattern of these trains often varies in development and disease. While the isoforms differ both in the number of sites of glycosylation and the types of carbohydrate attached to the protein, classical methods of glycan analysis are insensitive at the levels typically separated by 2-D PAGE. Developments in mass spectrometry technologies have enabled the characterization of most of the oligosaccharide attributes to be determined on picomole amounts of protein. These techniques are beginning to allow the glycoform heterogeneity on 2-D separated glycoproteins to be analyzed.

Original languageEnglish
Pages (from-to)1872-1882
Number of pages11
JournalElectrophoresis
Volume19
Issue number11
DOIs
Publication statusPublished - Aug 1998

Keywords

  • glycoprotein
  • mass spectrometry
  • proteome
  • two-dimensional polyacrylamide gel electrophoresis

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