GlycoDigest: a tool for the targeted use of exoglycosidase digestions in glycan structure determination

Lou Gotz, Jodie L. Abrahams, Julien Mariethoz, Pauline M. Rudd, Niclas G. Karlsson, Nicolle H. Packer, Matthew P. Campbell*, Frederique Lisacek

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Sequencing oligosaccharides by exoglycosidases, either sequentially or in an array format, is a powerful tool to unambiguously determine the structure of complex N- and O-link glycans. Here, we introduce GlycoDigest, a tool that simulates exoglycosidase digestion, based on controlled rules acquired from expert knowledge and experimental evidence available in GlycoBase. The tool allows the targeted design of glycosidase enzyme mixtures by allowing researchers to model the action of exoglycosidases, thereby validating and improving the efficiency and accuracy of glycan analysis.

Original languageEnglish
Pages (from-to)3131-3133
Number of pages3
JournalBioinformatics
Volume30
Issue number21
DOIs
Publication statusPublished - 1 Nov 2014

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