GlycoDigest: a tool for the targeted use of exoglycosidase digestions in glycan structure determination

Lou Gotz; Jodie L. Abrahams; Julien Mariethoz; Pauline M. Rudd; Niclas G. Karlsson; Nicolle H. Packer; Matthew P. Campbell; Frederique Lisacek

doi:10.1093/bioinformatics/btu425

GlycoDigest: a tool for the targeted use of exoglycosidase digestions in glycan structure determination

Lou Gotz, Jodie L. Abrahams, Julien Mariethoz, Pauline M. Rudd, Niclas G. Karlsson, Nicolle H. Packer, Matthew P. Campbell^*, Frederique Lisacek

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Sequencing oligosaccharides by exoglycosidases, either sequentially or in an array format, is a powerful tool to unambiguously determine the structure of complex N- and O-link glycans. Here, we introduce GlycoDigest, a tool that simulates exoglycosidase digestion, based on controlled rules acquired from expert knowledge and experimental evidence available in GlycoBase. The tool allows the targeted design of glycosidase enzyme mixtures by allowing researchers to model the action of exoglycosidases, thereby validating and improving the efficiency and accuracy of glycan analysis.

Original language	English
Pages (from-to)	3131-3133
Number of pages	3
Journal	Bioinformatics
Volume	30
Issue number	21
DOIs	https://doi.org/10.1093/bioinformatics/btu425
Publication status	Published - 1 Nov 2014

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abstract = "Sequencing oligosaccharides by exoglycosidases, either sequentially or in an array format, is a powerful tool to unambiguously determine the structure of complex N- and O-link glycans. Here, we introduce GlycoDigest, a tool that simulates exoglycosidase digestion, based on controlled rules acquired from expert knowledge and experimental evidence available in GlycoBase. The tool allows the targeted design of glycosidase enzyme mixtures by allowing researchers to model the action of exoglycosidases, thereby validating and improving the efficiency and accuracy of glycan analysis.",

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AU - Gotz, Lou

AU - Abrahams, Jodie L.

AU - Mariethoz, Julien

AU - Rudd, Pauline M.

AU - Karlsson, Niclas G.

AU - Packer, Nicolle H.

AU - Campbell, Matthew P.

AU - Lisacek, Frederique

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AB - Sequencing oligosaccharides by exoglycosidases, either sequentially or in an array format, is a powerful tool to unambiguously determine the structure of complex N- and O-link glycans. Here, we introduce GlycoDigest, a tool that simulates exoglycosidase digestion, based on controlled rules acquired from expert knowledge and experimental evidence available in GlycoBase. The tool allows the targeted design of glycosidase enzyme mixtures by allowing researchers to model the action of exoglycosidases, thereby validating and improving the efficiency and accuracy of glycan analysis.

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JO - Bioinformatics

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ER -

GlycoDigest: a tool for the targeted use of exoglycosidase digestions in glycan structure determination

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