GlycoMob: an ion mobility-mass spectrometry collision cross section database for glycomics

Weston B. Struwe*, Kevin Pagel, Justin L. P. Benesch, David J. Harvey, Matthew P. Campbell

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

Ion mobility mass spectrometry (IM-MS) is a promising analytical technique for glycomics that separates glycan ions based on their collision cross section (CCS) and provides glycan precursor and fragment masses. It has been shown that isomeric oligosaccharide species can be separated by IM and identified on basis of their CCS and fragmentation. These results indicate that adding CCSs information for glycans and glycan fragments to searchable databases and analysis pipelines will increase identification confidence and accuracy. We have developed a freely accessible database, GlycoMob (http://www.glycomob.org), containing over 900 CCSs values of glycans, oligosaccharide standards and their fragments that will be continually updated. We have measured the absolute CCSs of calibration standards, biologically derived and synthetic N-glycans ionized with various adducts in positive and negative mode or as protonated (positive ion) and deprotonated (negative ion) ions.

Original languageEnglish
Pages (from-to)399-404
Number of pages6
JournalGlycoconjugate Journal
Volume33
Issue number3
DOIs
Publication statusPublished - Jun 2016

Keywords

  • database
  • glycoinformatics
  • ion mobility
  • mass spectrometry
  • glycomics
  • glycan

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