Glycoprotein detection of 2-D separated proteins

Nicolle H. Packer*, Malcolm S. Ball, Peter L. Devine

*Corresponding author for this work

Research output: Contribution to journalReview article

23 Citations (Scopus)

Abstract

The profile of the two-dimensional (2-D) separation of the protein complement (proteome) of eukaryotic cells and tissues typically contains obvious “trains” of spots that differ in pI and/or apparent molecular mass. These are usually isoforms of the same protein and result from a variety of posttranslational modifications. There is growing evidence that alterations to the glycosylation of a protein can be correlated with developmental and pathological changes; these changes can be visualized on the 2-D separation by alterations in the pattern of these glycoprotein isoforms.
Original languageEnglish
Pages (from-to)341-352
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume112
DOIs
Publication statusPublished - 1999

Fingerprint Dive into the research topics of 'Glycoprotein detection of 2-D separated proteins'. Together they form a unique fingerprint.

  • Cite this