Glycosylation and functional activity of anti-D secreted by two human lymphoblastoid cell lines

Diane Cant*, John Barford, Colin Harbour, Anne Fletcher, Nicole Packer, Andrew Gooley

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cell lines BTSN4 and BTSN5 were produced by the Epstein-Barr Virus (EBV) transformation of B-lymphocytes from the same human donor. Both secrete an anti-D monoclonal of the IgG1 subclass but these antibodies display vastly different effector activities. Specifically, anti-D from BTSN4 has a far greater activity in both monocyte-and lymphocyte-mediated ADCC reactions and causes a higher percentage of rosettes to be formed with monocyte-like U937 cells. This variation in functional activity is shown to coincide with changes in the structure of the sugar chains attached to the asparagine-297 site on the immunoglobulin heavy chain.

Original languageEnglish
Pages (from-to)223-228
Number of pages6
JournalCytotechnology
Volume15
Issue number1-3
DOIs
Publication statusPublished - Feb 1994

Keywords

  • anti-D
  • effector function
  • glycosylation
  • immunoglobulin G
  • monoclonal antibody

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