Glycosylhydrazines, preparation, immobilization and reactions of: glycoprotein analysis and O-glycan removal

John William Redmond (Inventor), Nicolle Hannah Packer (Inventor), Andrew Arthur Gooley (Inventor), Keith Leslie Williams (Inventor), Michael Batley (Inventor), Warren Charles Kerr (Inventor), Anthony Pisano (Inventor), Helen Joan Tweeddale (Inventor), Catherine Anne Cooper (Inventor)

Research output: Patent

Abstract

The present invention consists in methods of preparing sugar derivatives either in isolation or from glycopeptides or glycoproteins. The methods comprise producing sugar hydrazones, sugar pyrazoles, glycosylpyrazones, azoglycan dyes and hydrazoglycan dyes. The present invention also relates to the removal of O-glycans from glycopeptides or glycoproteins, immobilizing reducing sugars onto solid supports and methods to obtain sugars from a glycopeptide or glycoprotein comprising subjecting the glycopeptide or glycoprotein to solid phase Edman degradation followed by separating and characterizing the sugars.
Original languageEnglish
Patent numberUS5831077
IPCC07H 5/06,C07H 1/00,C07H 1/08,C07H 9/00,C07H 9/06,C07H 11/00,C07K 1/00,C07K 1/12,C09B 29/00,C09B 29/50
Priority date9/12/93
Publication statusPublished - 3 Nov 1998

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