The present invention consists in methods of preparing sugar derivatives either in isolation or from glycopeptides or glycoproteins. The methods comprise producing sugar hydrazones, sugar pyrazoles, glycosylpyrazones, azoglycan dyes and hydrazoglycan dyes. The present invention also relates to the removal of O-glycans from glycopeptides or glycoproteins, immobilising reducing sugars onto solid supports and methods to obtain sugars from a glycopeptide or glycoprotein comprising subjecting the glycopeptide or glycoprotein to solid-phase Edman degradation followed by separating and characterising the sugars.
|IPC||C07H 1/00,C07H 5/04,C07H 13/04,C07H 15/26,C07H 19/04,C07K 1/107,C07K 1/12,C08B 37/02,C08J 7/12,C09B 29/46,C09B 29/50,C09B 39/00,C09B 55/00,G01N 33/00,G01N 33/68|
|Publication status||Published - 8 Jul 1999|