Granulocyte macrophage colony stimulating factor stimulates in vitro proliferation of astrocytes derived from simian mature brains

Gilles Guillemin, François D. Boussin*, Roger Le Grand, Juliana Croitoru, Hervé Coffigny, Dominique Dormont

*Corresponding author for this work

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

In the brain, granulocyte-macrophage colony stimulating factor (GM-CSF) may be released by infiltrated cells of the immune system including T and B lymphocytes and mononuclear phagocytes, but also by nervous system resident cells such as microglia and astrocytes. Astrocyte-secreted GM-CSF may play an important role in enhancing the local inflammatory response to central nervous system (CNS) injury and in recruting microglia and activated macrophages. In this study, we demonstrated that GM-CSF, as TNFα and IL 6, stimulates in vitro proliferation of simian astrocytes in primary cultures. Results were confirmed by blocking experiments performed with a specific neutralizing mAb directed against GM-CSF. Furthermore, we demonstrated that GM-CSF mediates its effect on these cells through the α subunit of the GM- CSF receptor which is constitutively expressed at the membrane of the cultured simian astrocytes as assessed by immunofluorescence. GM-CSF effects on astrocytes could be involved in astrocytosis, a hallmark of various neurological injuries and in inflammatory processes in an autocrine manner.

Original languageEnglish
Pages (from-to)71-80
Number of pages10
JournalGLIA
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 1996
Externally publishedYes

Keywords

  • Granulocyte-macrophage colony stimulating factor
  • Simian primary astrocytes
  • Tumor necrosis factor-α

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