Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

Sam Abraham, James Chin, Huub J. M. Brouwers, Bernadette Turner, Ren Zhang, Toni A. Chapman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).

Original languageEnglish
Pages (from-to)6691-6693
Number of pages3
JournalApplied and Environmental Microbiology
Volume77
Issue number18
DOIs
Publication statusPublished - Sep 2011
Externally publishedYes

Keywords

  • ESCHERICHIA-COLI
  • BACTERIOCINS
  • PROMOTERS

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