Abstract
Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).
Original language | English |
---|---|
Pages (from-to) | 6691-6693 |
Number of pages | 3 |
Journal | Applied and Environmental Microbiology |
Volume | 77 |
Issue number | 18 |
DOIs | |
Publication status | Published - Sept 2011 |
Externally published | Yes |
Keywords
- ESCHERICHIA-COLI
- BACTERIOCINS
- PROMOTERS