Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

Sam Abraham; James Chin; Huub J. M. Brouwers; Bernadette Turner; Ren Zhang; Toni A. Chapman

doi:10.1128/AEM.00534-11

Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

Sam Abraham, James Chin, Huub J. M. Brouwers, Bernadette Turner, Ren Zhang, Toni A. Chapman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).

Original language	English
Pages (from-to)	6691-6693
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	77
Issue number	18
DOIs	https://doi.org/10.1128/AEM.00534-11
Publication status	Published - Sept 2011
Externally published	Yes

Keywords

ESCHERICHIA-COLI
BACTERIOCINS
PROMOTERS

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10.1128/AEM.00534-11

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title = "Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins",

abstract = "Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).",

keywords = "ESCHERICHIA-COLI, BACTERIOCINS, PROMOTERS",

author = "Sam Abraham and James Chin and Brouwers, \{Huub J. M.\} and Bernadette Turner and Ren Zhang and Chapman, \{Toni A.\}",

year = "2011",

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doi = "10.1128/AEM.00534-11",

language = "English",

volume = "77",

pages = "6691--6693",

journal = "Applied and Environmental Microbiology",

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publisher = "American Society for Microbiology",

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T1 - Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

AU - Abraham, Sam

AU - Chin, James

AU - Brouwers, Huub J. M.

AU - Turner, Bernadette

AU - Zhang, Ren

AU - Chapman, Toni A.

PY - 2011/9

Y1 - 2011/9

N2 - Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).

AB - Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).

KW - ESCHERICHIA-COLI

KW - BACTERIOCINS

KW - PROMOTERS

UR - https://www.scopus.com/pages/publications/80052816350

U2 - 10.1128/AEM.00534-11

DO - 10.1128/AEM.00534-11

M3 - Article

C2 - 21803919

SN - 0099-2240

VL - 77

SP - 6691

EP - 6693

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 18

ER -

Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

Abstract

Keywords

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