Green porous benzamide-like nanomembranes for hazardous cations detection, separation, and concentration adjustment

Navid Rabiee*, Yousef Fatahi, Mohsen Asadnia*, Hossein Daneshgar, Mahsa Kiani, Amir Mohammad Ghadiri, Monireh Atarod, Amin Hamed Mashhadzadeh, Omid Akhavan, Mojtaba Bagherzadeh, Eder C. Lima, Mohammad Reza Saeb

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Green biomaterials play a crucial role in the diagnosis and treatment of diseases as well as health-related problem-solving. Typically, biocompatibility, biodegradability, and mechanical strength are requirements centered on biomaterial engineering. However, in-hospital therapeutics require an elaborated synthesis of hybrid and complex nanomaterials capable of mimicking cellular behavior. Accumulation of hazardous cations like K+ in the inner and middle ear may permanently damage the ear system. We synthesized nanoplatforms based on Allium noeanum to take the first steps in developing biological porous nanomembranes for hazardous cation detection in biological media. The 1,1,1-tris[[(2′-benzyl-amino-formyl)phenoxy]methyl]ethane (A), 4-amino-benzo-hydrazide (B), and 4-(2-(4-(3-carboxy-propan-amido)benzoyl)hydrazineyl)-4-oxobutanoic acid (B1) were synthesized to obtain green ligands based on 4-X-N-(…(Y(hydrazine-1-carbonyl)phenyl)benzamide, with X denoting fluoro (B2), methoxy (B3), nitro (B4), and phenyl-sulfonyl (B5) substitutes. The chemical structure of ligand-decorated adenosine triphosphate (ATP) molecules (S-ATP) was characterized by FTIR, XRD, AFM, FESEM, and TEM techniques. The cytotoxicity of the porous membrane was patterned by applying different cell lines, including HEK-293, PC12, MCF-7, HeLa, HepG2, and HT-29, to disclose their biological behavior. The morphology of cultured cells was monitored by confocal laser scanning microscopy. The sensitivity of S-ATP to different cations of Na+, Mg2+, K+, Ba2+, Zn2+, and Cd2+ was evaluated by inductively coupled plasma atomic emission spectroscopy (ICP-AES) in terms of extraction efficiency (η). For pH of 5.5, the η of A-based S-ATP followed the order Na+ (63.3%) > Mg2+ (62.1%) > Ba2+ (7.6%) > Ca2+ (5.5%); while for pH of 7.4, Na+ (37.0%) > Ca2+ (33.1%) > K+ (25.7%). The heat map of MTT and dose-dependent evaluations unveiled acceptable cell viability of more than 90%. The proposed green porous nanomembranes would pave the way to use multifunctional green porous nanomembranes in biological membranes.

Original languageEnglish
Article number127130
Pages (from-to)1-18
Number of pages18
JournalJournal of Hazardous Materials
Issue numberPart B
Early online date11 Sep 2021
Publication statusE-pub ahead of print - 11 Sep 2021


  • Green chemistry
  • Membrane
  • Green synthesis
  • Cell viability
  • Biological properties


Dive into the research topics of 'Green porous benzamide-like nanomembranes for hazardous cations detection, separation, and concentration adjustment'. Together they form a unique fingerprint.

Cite this