Growth and production of xylanolytic enzymes by the extreme thermophilic anaerobic bacterium Thermotoga thermarum

A. Sunna, G. Antranikian

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Cultivation of the extreme thermophilic anaerobic bacterium Thermotoga thermarum at 77°C on xylan was accompanied by the formation of heat-stable endoxylanase (136 U/l), β-xylosidase (44 U/l) and α-arabinofuranosidase (10 U/l). These enzymes were mainly associated with the cells and could not be released by detergent treatment {0.1-1.0 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS)}. Endoxylanases with a molecular weight of 40, 83 and 100 kDa were induced when xylan or xylose were used as substrates for growth. In the presence of other sugars like glucose, maltose, arabinose or starch, low concentrations of the low-molecular-weight endoxylanase (40 kDa) was detected. Xylose was found to be the best substrate for the induction of α-xylosidase and α-arabinofuranosidase but not for growth. Cultivation of T. thermarum in a dialysis batch fermenter resulted in a significant increase in cell concentration and enzyme level. A total cell count of 1.3 x 10 9 cells/ml and 202 U/l of endoxylanase were measured when partially soluble birchwood xylan was used as the carbon source. The use of insoluble birchwood xylan as the substrate caused the elevation of the maximal cell concentration and enzyme level up to 2.0 x 10 9 cells/ml and 540 U/l, respectively.

LanguageEnglish
Pages671-676
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume45
Issue number5
DOIs
Publication statusPublished - 1996
Externally publishedYes

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Endo-1,4-beta Xylanases
Xylans
Anaerobic Bacteria
Bacteria
Enzymes
Xylose
Growth
Substrates
Molecular weight
Xylosidases
Fermenters
Maltose
Arabinose
Molecular Weight
Dialysis
Detergents
Starch
Sugars
Glucose
Carbon

Cite this

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title = "Growth and production of xylanolytic enzymes by the extreme thermophilic anaerobic bacterium Thermotoga thermarum",
abstract = "Cultivation of the extreme thermophilic anaerobic bacterium Thermotoga thermarum at 77°C on xylan was accompanied by the formation of heat-stable endoxylanase (136 U/l), β-xylosidase (44 U/l) and α-arabinofuranosidase (10 U/l). These enzymes were mainly associated with the cells and could not be released by detergent treatment {0.1-1.0 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS)}. Endoxylanases with a molecular weight of 40, 83 and 100 kDa were induced when xylan or xylose were used as substrates for growth. In the presence of other sugars like glucose, maltose, arabinose or starch, low concentrations of the low-molecular-weight endoxylanase (40 kDa) was detected. Xylose was found to be the best substrate for the induction of α-xylosidase and α-arabinofuranosidase but not for growth. Cultivation of T. thermarum in a dialysis batch fermenter resulted in a significant increase in cell concentration and enzyme level. A total cell count of 1.3 x 10 9 cells/ml and 202 U/l of endoxylanase were measured when partially soluble birchwood xylan was used as the carbon source. The use of insoluble birchwood xylan as the substrate caused the elevation of the maximal cell concentration and enzyme level up to 2.0 x 10 9 cells/ml and 540 U/l, respectively.",
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Growth and production of xylanolytic enzymes by the extreme thermophilic anaerobic bacterium Thermotoga thermarum. / Sunna, A.; Antranikian, G.

In: Applied Microbiology and Biotechnology, Vol. 45, No. 5, 1996, p. 671-676.

Research output: Contribution to journalArticleResearchpeer-review

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