The ocular circulation provides readily visible information about the state of the systemic circulation, as well as being potentially of relevance to the pathogenesis of ocular disorders such as glaucoma. The interaction between intraocular pressure, retinal vessels and cerebrospinal fluid pressure located at the retrolaminar portion of the eye has been of great interest for both ophthalmic and neurological clinicians and researchers. Understanding the relationship between these physiological parameters can explain phenomena such as spontaneous retinal venous pulsatility, and characterize the effects of the translaminar pressure gradient. It may be feasible to use measurable changes in venous pulsatility to enhance clinical assessment in different diseases. In this article we review recent findings on ocular hemodynamics and the relevance of these parameters in the diagnosis of ophthalmic and neurological diseases.