Heterologous gene expression in filamentous fungi: a holistic view

Helena Nevalainen*, Valentino Te'o, Merja Penttilä, Tiina Pakula

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

11 Citations (Scopus)

Abstract

As scavengers of recalcitrant polymers in the nature, filamentous fungi are excellent secretors of proteins outside the growing mycelium. This characteristic has been targeted and systematically improved in industrially-exploited production strains. Over the last five years there has been a significant shift from one-gene-at-a-time approaches to wider understanding of the organism, made possible, for example by gene array and proteome technologies that can now also be applied to filamentous fungi. This has presented novel opportunities for studies into gene regulation under specific conditions such as a particular carbon source or developmental stage with a view of advancing the basic knowledge and gaining information that can be applied for strain and process improvement. Filamentous fungi offer enormous potential for efficient and large scale production of heterologous gene products. Importantly, protein secretion provides a platform for the eukaryotic style posttranslational modification of gene products. Fungi are cheap to cultivate and down-stream processing is made easy with no need to break cells open for product recovery. In order to capitalize on fungi as heterologous production hosts, research is now directed to revealing the cellular mechanisms for internal protein quality control, secretion stress, functional genomics of protein expression and secretion, protein modification and linking the physiology to productivity.

Original languageEnglish
Title of host publicationApplied Mycology and Biotechnology
Subtitle of host publicationGenes and Genomics
EditorsDilip K. Arora, Randy M. Berka
Place of PublicationAmsterdam
PublisherElsevier
Pages211-237
Number of pages27
Volume5
ISBN (Print)9780444518088
DOIs
Publication statusPublished - 2005

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